Genetic analysis of human immunodeficiency virus type 1 and 2 (HIV-1 and HIV-2) mixed infections in India reveals a recent spread of HIV-1 and HIV-2 from a single ancestor for each of these viruses
Genetic analysis of human immunodeficiency virus type 1 and 2 (HIV-1 and HIV-2) mixed infections in India reveals a recent spread of HIV-1 and HIV-2 from a single ancestor for each of these viruses
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对印度 1 型和 2 型人类免疫缺陷病毒（HIV-1 和 HIV-2）混合感染的遗传分析表明，最近 HIV-1 和 HIV-2 的传播均来自这些病毒的单一祖先
作者：
M. Grez;Ursula Dietrich;P. Balfe;H. V. Briesen;K. Maniar;G. Mahambre;E. Delwart;James I. Mullins;Helga RUBSAMEN
从印度孟买血清学确定的HIV-1/HIV-2混合感染患者的未培养外周血单个核细胞中，用PCR扩增了编码人类免疫缺陷病毒1型（HIV-1）和2型（HIV-2）表面包膜糖蛋白的DNA序列。在7例随机选择的双反应性病例中，有7例获得了hiv -1特异性PCR产物，而在7例中有5例（71%）检测到hiv -2特异性序列。DNA序列分析表明，HIV-1 gp120编码序列彼此密切相关（核苷酸序列差异在3.1 ~ 6.8%之间）。系统发育树分析将印度毒株置于HIV-1的C亚型，与之前在东非和南非发现的序列最相似。HIV-2序列也彼此密切相关，总体序列差异在5.6%至10.5%之间。印度HIV-1和HIV-2序列之间的核苷酸差异水平较低，这表明在每种情况下，每种病毒都是在相当短的时间内从单一入口点引入这一人群的。本文报道的HIV-2序列代表了对亚洲HIV-2毒株的首次分析，并证实了先前在印度检测到的血清学模式。这些数据表明，HIV-2和HIV-1的大量传播已经出现在非洲以外迄今未接触过HIV的人群中。这些发现意味着HIV-2在世界范围内的进一步传播是可以预期的，并且对未来的疫苗和治疗开发具有重要意义。
DNA sequences encoding the surface envelope glycoproteins of human immunodeficiency virus type 1 (HIV-1) and type 2 (HIV-2) were amplified by PCR from uncultured peripheral blood mononuclear cells obtained from patients with serologically defined HIV-1/HIV-2 mixed infections from Bombay, India. HIV-1-specific PCR products were obtained in seven of seven randomly chosen doubly reactive cases, while HIV-2-specific sequences were detected in five of seven cases (71%). DNA sequence analysis showed that the HIV-1 gp120 coding sequences were closely related to each other (nucleotide sequence divergence of between 3.1 and 6.8%). Phylogenetic tree analysis placed the Indian strains within the C subtype of HIV-1, being most similar to sequences previously found in East and South Africa. The HIV-2 sequences were also closely related to each other, with an overall sequence divergence of between 5.6 and 10.5%. The low level of nucleotide divergence among Indian HIV-1 and HIV-2 sequences suggests a fairly recent introduction of each virus into this population from a single point of entry in each case. The HIV-2 sequences reported here represent the first analysis of Asian HIV-2 strains and confirm the serological pattern previously detected in India. These data show that a substantial spread of HIV-2, together with HIV-1, has appeared outside Africa in a population hitherto unexposed to HIV. These findings imply that further spread of HIV-2 worldwide is to be expected and have important implications for future vaccine and therapy development.
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影响因子：
56.9
作者：
WOLINSKY, SM;WIKE, CM;MUNOZ, JL
通讯作者：
MUNOZ, JL
DOI：
10.1073/pnas.88.24.11236
发表时间：
1991-12-01
影响因子：
11.1
作者：
BURGER, H;WEISER, B;GIBBS, RA
通讯作者：
GIBBS, RA
对暴露于人类免疫缺陷病毒 2 型 (HIV-2) 的个体进行临床、血液学和免疫学横断面评估。
影响因子：
1.5
作者：
Marlink,RG;Ricard,D;M'Boup,S;Kanki,PJ;Romet-Lemonne,JL;N'Doye,I;Diop,K;Simpson,MA;Greco,F;Chou,MJ
通讯作者：
Chou,MJ
影响因子：
158.5
作者：
DAAR, ES;MOUDGIL, T;HO, DD
通讯作者：
HO, DD