Increase in the Intracellular Bulk Water Content in the Early Phase of Cell Death of Keratinocytes, Corneoptosis, as Revealed by 65 GHz Near-Field CMOS Dielectric Sensor.
Increase in the Intracellular Bulk Water Content in the Early Phase of Cell Death of Keratinocytes, Corneoptosis, as Revealed by 65 GHz Near-Field CMOS Dielectric Sensor.
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DOI：
10.3390/molecules27092886
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发表时间：
2022-04-30
期刊：
影响因子：
4.6
通讯作者：
Matsui, Takeshi
中科院分区：
文献类型：
--
作者：
Shiraga, Keiichiro;Ogawa, Yuichi;Kikuchi, Shojiro;Amagai, Masayuki;Matsui, Takeshi
虽然本体水和水合水共存于细胞中以支持生物大分子的表达，但由于缺乏评估技术，迄今为止几乎没有探索水分子的动力学如何在分子生物学研究中仅起次要作用，与细胞状态的变化（如细胞死亡）相关。在这项研究中，我们开发了一个高精度的测量系统，可以区分散装水含量的变化±0.02%（0.2 mg/cm 3）与单细胞水平的空间分辨率的基础上，近场CMOS介电传感器工作在65 GHz。我们应用这个系统来评估角质形成细胞的细胞死亡过程中，称为角质形成，使用分离的SG 1（第一层颗粒层）细胞在体外的体积水含量的时间变化。在角膜下垂过程中，在膜破裂前约1小时观察到大量水含量的显著不可逆增加，这始于细胞质高Ca 2+信号。这些发现表明，钙流可能有一个触发的SG 1细胞中的体积水含量的增加的作用。因此，我们的近场CMOS介电传感器提供了一个有价值的工具，以剖析水分子在细胞中发生的各种事件的参与。
While bulk water and hydration water coexist in cells to support the expression of biological macromolecules, how the dynamics of water molecules, which have long been only a minor role in molecular biology research, relate to changes in cellular states such as cell death has hardly been explored so far due to the lack of evaluation techniques. In this study, we developed a high-precision measurement system that can discriminate bulk water content changes of ±0.02% (0.2 mg/cm3) with single-cell-level spatial resolution based on a near-field CMOS dielectric sensor operating at 65 GHz. We applied this system to evaluate the temporal changes in the bulk water content during the cell death process of keratinocytes, called corneoptosis, using isolated SG1 (first layer of stratum granulosum) cells in vitro. A significant irreversible increase in the bulk water content was observed approximately 1 h before membrane disruption during corneoptosis, which starts with cytoplasmic high Ca2+ signal. These findings suggest that the calcium flux may have a role in triggering the increase in the bulk water content in SG1 cells. Thus, our near-field CMOS dielectric sensor provides a valuable tool to dissect the involvement of water molecules in the various events that occur in the cell.
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DOI：
10.1074/jbc.m111.329219
发表时间：
2012-03-30
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Conner MT;Conner AC;Bland CE;Taylor LH;Brown JE;Parri HR;Bill RM
通讯作者：
Bill RM
影响因子：
3.3
作者：
Li, Z;Lazaridis, T
通讯作者：
Lazaridis, T
影响因子：
5.4
作者：
Mitsunaka, Takeshi;Sato, Daiki;Fujishima, Minoru
通讯作者：
Fujishima, Minoru
影响因子：
5.5
作者：
Bortner CD;Cidlowski JA
通讯作者：
Cidlowski JA
影响因子：
4.6
作者：
Martins, Murillo L.;Dinitzen, Alexander B.;Bordallo, Heloisa N.
通讯作者：
Bordallo, Heloisa N.