Candida albicans Cdc15 is essential for mitotic exit and cytokinesis.
Candida albicans Cdc15 is essential for mitotic exit and cytokinesis.
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DOI：
10.1038/s41598-018-27157-y
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发表时间：
2018-06-11
影响因子：
4.6
通讯作者：
Bates S
中科院分区：
文献类型：
--
作者：
Bates S
白色念珠菌表现出多种形态，而转换形态的能力必须与细胞周期控制相关。在芽殖酵母中，有丝分裂退出网络 (MEN) 的作用是驱动有丝分裂退出并发出胞质分裂和细胞分离的信号。然而，之前关于白色念珠菌 MEN 的报告对其在该生物体中的作用提出了质疑，迄今为止分析的成分表明在有丝分裂退出、胞质分裂和细胞分离过程中的重要性不同。这项工作重点关注 Cdc15 激酶在白色念珠菌中的作用，并证明与酿酒酵母类似，它在有丝分裂退出和胞质分裂的信号传导中发挥重要作用。 Cdc15耗尽的细胞发育成细长的丝，这是白色念珠菌细胞周期停滞的常见反应。这些细丝仅从大芽细胞中出现，包含两个核体并表现出超延伸的纺锤体，这些细胞的所有特征都未能退出有丝分裂。此外，这些丝显示出明显的胞质分裂缺陷，CDC15 过度表达导致菌丝形态发生后异常的细胞分离。总之，这些结果与 Cdc15 在白色念珠菌有丝分裂退出、胞质分裂和细胞分离信号传导中发挥重要作用是一致的。
Candida albicans displays a variety of morphological forms, and the ability to switch forms must be linked with cell cycle control. In budding yeast the Mitotic Exit Network (MEN) acts to drive mitotic exit and signal for cytokinesis and cell separation. However, previous reports on the MEN in C. albicans have raised questions on its role in this organism, with the components analysed to date demonstrating differing levels of importance in the processes of mitotic exit, cytokinesis and cell separation. This work focuses on the role of the Cdc15 kinase in C. albicans and demonstrates that, similar to Saccharomyces cerevisiae, it plays an essential role in signalling for mitotic exit and cytokinesis. Cells depleted of Cdc15 developed into elongated filaments, a common response to cell cycle arrest in C. albicans. These filaments emerged exclusively from large budded cells, contained two nuclear bodies and exhibited a hyper-extended spindle, all characteristic of these cells failing to exit mitosis. Furthermore these filaments displayed a clear cytokinesis defect, and CDC15 over-expression led to aberrant cell separation following hyphal morphogenesis. Together, these results are consistent with Cdc15 playing an essential role in signalling for mitotic exit, cytokinesis and cell separation in C. albicans.
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