Molecular interaction of 1-aminocyclopropane-1-carboxylate deaminase (ACCD)-producing endophytic Streptomyces sp. GMKU 336 towards salt-stress resistance of Oryza sativa L. cv. KDML105.
Molecular interaction of 1-aminocyclopropane-1-carboxylate deaminase (ACCD)-producing endophytic Streptomyces sp. GMKU 336 towards salt-stress resistance of Oryza sativa L. cv. KDML105.
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DOI：
10.1038/s41598-018-19799-9
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发表时间：
2018-01-31
影响因子：
4.6
通讯作者：
Thamchaipenet A
中科院分区：
文献类型：
--
作者：
Jaemsaeng R;Jantasuriyarat C;Thamchaipenet A
将产1-氨基环丙烷-1-羧酸脱氨酶（ACCD）内生菌Streptomyces sp. GMKU 336及其缺乏ACCD的突变体接种到泰国茉莉品种Khao Dok Mali 105上。KDML105)在盐胁迫（150 mM NaCl）条件下。结果表明，链霉菌GMKU 336对植株生长、叶绿素、脯氨酸、K+、Ca+和水分含量均有显著的促进作用；但与未接种和接种了accd缺陷突变体的植株相比，乙烯、活性氧（ROS）、Na+和Na+/K+比值降低。水稻GMKU 336相关胁迫响应基因的表达谱与植株生理特性相关。参与乙烯通路的基因ACO1和EREBP1显著下调；而Streptomyces sp. GMKU 336编码ACCD的acdS在体内表达上调。此外，参与渗透平衡的基因（BADH1）、Na+转运蛋白（NHX1和SOS1）、钙调蛋白（Cam1-1）和抗氧化酶（CuZn-SOD1和CATb）均上调；而与信号级联有关的基因MAPK5则下调。本研究首次证明了产ACCD链霉菌GMKU 336通过ACCD的作用减少乙烯，从而促进水稻的生长和耐盐性，并进一步帮助植物清除活性氧，平衡离子含量和渗透压。
1-aminocyclopropane-1-carboxylate deaminase (ACCD)-producing endophytic Streptomyces sp. GMKU 336 and its ACCD-deficient mutant were inoculated into Thai jasmine rice Khao Dok Mali 105 cultivar (Oryza sativa L. cv. KDML105) under salt stress (150 mM NaCl) conditions. The results clearly indicated that Streptomyces sp. GMKU 336 significantly increased plant growth, chlorophyll, proline, K+, Ca+, and water contents; but decreased ethylene, reactive oxygen species (ROS), Na+, and Na+/K+ ratio when compared to plants not inoculated and those inoculated with the ACCD-deficient mutant. Expression profiles of stress responsive genes in rice in association with strain GMKU 336 were correlated to plant physiological characteristics. Genes involved in the ethylene pathway, ACO1 and EREBP1, were significantly down-regulated; while acdS encoding ACCD in Streptomyces sp. GMKU 336 was up-regulated in vivo. Furthermore, genes involved in osmotic balance (BADH1), Na+ transporters (NHX1 and SOS1), calmodulin (Cam1-1), and antioxidant enzymes (CuZn-SOD1 and CATb) were up-regulated; whereas, a gene implicated in a signaling cascade, MAPK5, was down-regulated. This work demonstrates the first time that ACCD-producing Streptomyces sp. GMKU 336 enhances growth of rice and increases salt tolerance by reduction of ethylene via the action of ACCD and further assists plants to scavenge ROS, balance ion content and osmotic pressure.
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