Genetic Plurality of OXA/NDM-Encoding Features Characterized From Enterobacterales Recovered From Czech Hospitals.
Genetic Plurality of OXA/NDM-Encoding Features Characterized From Enterobacterales Recovered From Czech Hospitals.
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DOI：
10.3389/fmicb.2021.641415
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发表时间：
2021
影响因子：
5.2
通讯作者：
Bitar I
中科院分区：
文献类型：
--
作者：
Chudejova K;Kraftova L;Mattioni Marchetti V;Hrabak J;Papagiannitsis CC;Bitar I
本研究的目的是表征4种产NDM和OXA-48样碳青霉烯酶的肠球菌，这些碳青霉烯酶来自有旅行史或/和既往在国外住院的捷克患者。肺炎克雷伯氏菌分离株属于“高风险”克隆ST 147、ST 11和ST 15，而大肠埃希氏菌分离株被指定为ST 167。所有分离株均对大多数β-内酰胺类（包括碳青霉烯类）耐药，同时对粘菌素保持敏感性。此外，WGS数据分析显示，所有4种分离株均以不同组合共产生OXA-48型和NDM型碳青霉烯酶（Kpn 47733：blaNDM-5 + blaOXA-181; Kpn 50595：blaNDM-1 + blaOXA-181; Kpn 51015：blaNDM-1 + blaOXA-244; Eco 52418：blaNDM-5 + blaOXA-244）。在Kpn 51015中，blaOXA-244位于质粒p51015_OXA-244上，而相应的基因定位于E. coli Eco52418。另一方面，在分离株Kpn 47733的ColKP 3质粒上鉴定出blaOXA-181，而在Kpn 50595中鉴定出携带blaOXA-181的质粒为IncX 3-ColKP 3融合体。blaNDM-1基因存在于两种不同的质粒上，p51015_NDM-1属于新的IncH质粒组，p51015_NDM-1是IncFK 1-FIB复制子。此外，blaNDM-5被发现在两个IncFII质粒显示有限的核苷酸相似性彼此。在两种质粒中，blaNDM-5的遗传环境是相同的。最后，在所有四种产碳青霉烯酶的分离株中，鉴定了不同数量的额外复制子，其中一些与重要的耐药决定簇相关，如blaCTX-M-15、blaCTX-2和ermB。总之，本研究报告首次描述了从捷克医院分离的产OXA-244肠球菌。此外，我们的研究结果表明，在获得和传播的决定因素编码OXA/NDM碳青霉烯酶的遗传多样性。
The aim of this study was to characterize four Enterobacterales co-producing NDM- and OXA-48-like carbapenemases from Czech patients with travel history or/and previous hospitalization abroad. Klebsiella pneumoniae isolates belonged to “high risk” clones ST147, ST11, and ST15, while the Escherichia coli isolate was assigned to ST167. All isolates expressed resistance against most β-lactams, including carbapenems, while retaining susceptibility to colistin. Furthermore, analysis of WGS data showed that all four isolates co-produced OXA-48- and NDM-type carbapenemases, in different combinations (Kpn47733: blaNDM–5 + blaOXA–181; Kpn50595: blaNDM–1 + blaOXA–181; Kpn51015: blaNDM–1 + blaOXA–244; Eco52418: blaNDM–5 + blaOXA–244). In Kpn51015, the blaOXA–244 was found on plasmid p51015_OXA-244, while the respective gene was localized in the chromosomal contig of E. coli Eco52418. On the other hand, blaOXA–181 was identified on a ColKP3 plasmid in isolate Kpn47733, while a blaOXA–181-carrying plasmid being an IncX3-ColKP3 fusion was identified in Kpn50595. The blaNDM–1 gene was found on two different plasmids, p51015_NDM-1 belonging to a novel IncH plasmid group and p51015_NDM-1 being an IncFK1-FIB replicon. Furthermore, the blaNDM–5 was found in two IncFII plasmids exhibiting limited nucleotide similarity to each other. In both plasmids, the genetic environment of blaNDM–5 was identical. Finally, in all four carbapenemase-producing isolates, a diverse number of additional replicons, some of these associated with important resistance determinants, like blaCTX–M–15, arr-2 and ermB, were identified. In conclusion, this study reports the first description of OXA-244-producing Enterobacterales isolated from Czech hospitals. Additionally, our findings indicated the genetic plurality involved in the acquisition and dissemination of determinants encoding OXA/NDM carbapenemases.
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