Regional variations in the cellular, biochemical, and biomechanical characteristics of rabbit annulus fibrosus.
Regional variations in the cellular, biochemical, and biomechanical characteristics of rabbit annulus fibrosus.
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DOI：
10.1371/journal.pone.0091799
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Li B
中科院分区：
文献类型：
--
作者：
Li J;Liu C;Guo Q;Yang H;Li B
由于纤维环组织的内在异质性，纤维环（AF）的组织工程，基本的承载椎间盘组件，仍然具有挑战性。为了提供一组AF组织的表征数据，作为构建组织工程化AF的基准，我们分析了来自AF的各个径向区域的组织和细胞，即，内AF（iAF）、中AF（mAF）和外AF（oAF）。我们发现，兔AF的细胞，生化和生物力学特性存在径向梯度。具体而言，iAF细胞（iAFCs）具有最高的胶原蛋白-II和聚集蛋白聚糖基因表达，而oAF细胞（oAFCs）具有最高的胶原蛋白-I基因表达。从iAF、mAF到oAF，DNA、总胶原和I型胶原含量依次增加，而糖胺聚糖（GAG）和II型胶原含量下降。原代AFCs的细胞牵引力从iAFCs、mAFCs到oAFCs逐渐降低，分别为336.6±155.3、199.0±158.8和123.8± 76.1Pa。iAF、mAF和oAF的储能模量分别为0.032±0.002、2.121±0.656和4.130±0.159 MPa。这些测量建立了一组参考数据，用于使用方便且具有成本效益的兔模型对AF组织工程策略的功效进行功能评价，其结果可进一步转化为人类研究。
Tissue engineering of annulus fibrosus (AF), the essential load-bearing disc component, remains challenging due to the intrinsic heterogeneity of AF tissue. In order to provide a set of characterization data of AF tissue, which serve as the benchmark for constructing tissue engineered AF, we analyzed tissues and cells from various radial zones of AF, i.e., inner AF (iAF), middle AF (mAF), and outer AF (oAF), using a rabbit model. We found that a radial gradient in the cellular, biochemical, and biomechanical characteristics of rabbit AF existed. Specifically, the iAF cells (iAFCs) had the highest expression of collagen-II and aggrecan genes, while oAF cells (oAFCs) had the highest collagen-I gene expression. The contents of DNA, total collagen and collagen-I sequentially increased from iAF, mAF to oAF, while glycosaminoglycan (GAG) and collagen-II levels decreased. The cell traction forces of primary AFCs gradually decreased from iAFCs, mAFCs to oAFCs, being 336.6±155.3, 199.0±158.8, and 123.8±76.1 Pa, respectively. The storage moduli of iAF, mAF, and oAF were 0.032±0.002, 2.121±0.656, and 4.130±0.159 MPa, respectively. These measurements have established a set of reference data for functional evaluation of the efficacy of AF tissue engineering strategies using a convenient and cost-effective rabbit model, the findings of which may be further translated to human research.
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影响因子：
2.4
作者：
Li, Bin;Lin, Michael;Tang, Ying;Wang, Bing;Wang, James H. -C.
通讯作者：
Wang, James H. -C.
DOI：
10.3390/s101109948
发表时间：
2010
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Li B;Wang JH
通讯作者：
Wang JH
影响因子：
5.3
作者：
Feng, Gang;Yang, Xinlin;Li, Xudong
通讯作者：
Li, Xudong
影响因子：
4.9
作者：
Koepsell, Laura;Remund, Tyler;Deng, Ying
通讯作者：
Deng, Ying
影响因子：
3
作者：
Hutton, WC;Elmer, WA;Hair, GA
通讯作者：
Hair, GA