Effects of nanosecond pulse electric fields on cellular elasticity.
Effects of nanosecond pulse electric fields on cellular elasticity.
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DOI：
10.1016/j.micron.2015.01.004
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发表时间：
2015-05
期刊：
影响因子：
2.4
通讯作者：
Stacey, Michael
中科院分区：
文献类型：
--
作者：
Dutta, Diganta;Asmar, Anthony;Stacey, Michael
利用荧光显微镜和原子力显微镜（AFM）研究了低强度（15kV/cm）和高强度（60kv /cm）的60纳秒脉冲电场（nsPEF）对Jurkat细胞形态和膜弹性的影响。我们使用原子力显微镜对细胞进行了力位移测量，并计算了膜弹性的杨氏模量。不同的脉冲条件下观察到不同的效应。我们发现单个低场强的nsPEF不会引起任何明显的细胞骨架破坏，并且有轻微的形态学改变。有趣的是，力测量和杨氏模量计算显示膜弹性显著降低。单个高场强的nsPEF由于破坏肌动蛋白细胞骨架和可能由不可逆膜损伤引起的弹性显著降低而引起明显的形态学变化。我们认为细胞形态主要依赖于肌动蛋白细胞骨架的稳定，而弹性变化部分依赖于细胞骨架的完整性。
We investigated the effects of a single 60 nanosecond pulsed electric field (nsPEF) of low (15kV/cm) and high (60 kV/cm) field strengths on cellular morphology and membrane elasticity in Jurkat cells using fluorescent microscopy and atomic force microscopy (AFM). We performed force displacement measurements on cells using AFM and calculated the Young’s modulus for membrane elasticity. Differential effects were observed dependent upon pulsing conditions. We found that a single nsPEF of low field strength did not induce any apparent cytoskeletal breakdown and had minor morphological changes. Interestingly, force measurements and calculation of Young’s modulus showed a significant decrease in membrane elasticity. A single nsPEF of high field strength induced stark morphological changes due to disruption of the actin cytoskeleton and a marked decrease in elasticity likely caused by irreversible membrane damage. We suggest that the cellular morphology is mainly dependent on stabilization by the actin cytoskeleton, while the elasticity changes are partially dependent on the cytoskeletal integrity.
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影响因子：
2.4
作者：
Vasilkoski, Zlatko;Esser, Axel T.;Weaver, James C.
通讯作者：
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DOI：
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发表时间：
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影响因子：
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DOI：
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影响因子：
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作者：
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通讯作者：
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DOI：
10.1016/j.bbrc.2006.01.094
发表时间：
2006-03-24
影响因子：
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作者：
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