Genome analysis of a novel Group I alphabaculovirus obtained from Oxyplax ochracea.
Genome analysis of a novel Group I alphabaculovirus obtained from Oxyplax ochracea.
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从 Oxyplax ochracea 获得的新型 I 类甲杆病毒的基因组分析
DOI：
10.1371/journal.pone.0192279
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Wang M
中科院分区：
文献类型：
--
作者：
Wang J;Hou D;Wang Q;Kuang W;Zhang L;Li J;Shen S;Deng F;Wang H;Hu Z;Wang M
墨氏氧蚤（Oxyplax ochracea）是一种对农作物、森林和果树造成严重损害的害虫。采用罗氏454焦磷酸测序系统测定了奥克拉棘球蚴核多角体病毒（OxocNPV）的全基因组序列。OxocNPV具有113,971 bp的双链DNA （dsDNA）基因组，G+C含量为31.1%。预计有124个假定的开放阅读框（orf）编码长度为bb50个氨基酸且重叠最小的蛋白质，覆盖了整个基因组的92%。鉴定出6个杆状病毒典型同源区（hrs）。系统发育分析和基因奇偶图分析表明，OxocNPV属于I族甲型杆状病毒的“a”枝，与I族甲型杆状病毒最近的共同祖先接近。确定了三个独特的orf（在国家生物技术信息中心数据库中没有同源物）。有趣的是，OxocNPV缺乏三个与病毒DNA复制和RNA转录相关的辅助基因（lef7， ie-2和pcna）。此外，OxocNPV的多个基因（包括ie1和odv-e66）序列与其他杆状病毒有显著差异。然而，三维结构预测表明OxocNPV ODV-E66含有保守的催化残基，这意味着它可能具有与AcMNPV ODV-E66一样的多糖裂解酶活性。所有这些独特的特征表明OxocNPV代表了I群甲型杆状病毒谱系的一个新物种。
Oxyplax ochracea (Moore) is a pest that causes severe damage to a wide range of crops, forests and fruit trees. The complete genome sequence of Oxyplax ochracea nucleopolyhedrovirus (OxocNPV) was determined using a Roche 454 pyrosequencing system. OxocNPV has a double-stranded DNA (dsDNA) genome of 113,971 bp with a G+C content of 31.1%. One hundred and twenty-four putative open reading frames (ORFs) encoding proteins of >50 amino acids in length and with minimal overlapping were predicted, which covered 92% of the whole genome. Six baculoviral typical homologous regions (hrs) were identified. Phylogenetic analysis and gene parity plot analysis showed that OxocNPV belongs to clade “a” of Group I alphabaculoviruses, and it seems to be close to the most recent common ancestor of Group I alphabaculoviruses. Three unique ORFs (with no homologs in the National Center for Biotechnology Information database) were identified. Interestingly, OxocNPV lacks three auxiliary genes (lef7, ie-2 and pcna) related to viral DNA replication and RNA transcription. In addition, OxocNPV has significantly different sequences for several genes (including ie1 and odv-e66) in comparison with those of other baculoviruses. However, three dimensional structure prediction showed that OxocNPV ODV-E66 contain the conserved catalytic residues, implying that it might possess polysaccharide lyase activity as AcMNPV ODV-E66. All these unique features suggest that OxocNPV represents a novel species of the Group I alphabaculovirus lineage.
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影响因子：
4.4
作者：
Ardisson-Araújo DM;Pereira BT;Melo FL;Ribeiro BM;Báo SN;de A Zanotto PM;Moscardi F;Kitajima EW;Sosa-Gomez DR;Wolff JL
通讯作者：
Wolff JL
影响因子：
3.7
作者：
IJkel, WFJ;Westenberg, M;Zuidema, D
通讯作者：
Zuidema, D
影响因子：
3.7
作者：
Jehle, JA;Lange, M;Hauschild, W
通讯作者：
Hauschild, W
影响因子：
5
作者：
Hu, ZH;Arif, BM;Vlak, JM
通讯作者：
Vlak, JM
影响因子：
2.7
作者：
Hilton, Sally;Winstanley, Doreen
通讯作者：
Winstanley, Doreen