The vertebrate ancestral repertoire of visual opsins, transducin alpha subunits and oxytocin/vasopressin receptors was established by duplication of their shared genomic region in the two rounds of early vertebrate genome duplications.
The vertebrate ancestral repertoire of visual opsins, transducin alpha subunits and oxytocin/vasopressin receptors was established by duplication of their shared genomic region in the two rounds of early vertebrate genome duplications.
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DOI：
10.1186/1471-2148-13-238
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发表时间：
2013-11-02
影响因子：
3.4
通讯作者：
Larhammar D
中科院分区：
文献类型：
--
作者：
Lagman D;Ocampo Daza D;Widmark J;Abalo XM;Sundström G;Larhammar D
脊椎动物的色觉依赖于四种主要的色视蛋白亚型：RH2(绿色视蛋白)、SWS1(紫外线视蛋白)、SWS2(蓝视蛋白)和LWS(红色视蛋白)。它们与弱光受体视紫红质(RH1)一起形成了脊椎动物视觉视蛋白家族。脊椎动物基因组包含许多多成员基因家族，这在很大程度上可以用脊椎动物祖先(2R)的两轮全基因组复制(WGD)和硬骨鱼祖先(3R)的第三轮全基因组复制(WGD)来解释。由WGD或区块复制产生的相关染色体区域被认为形成了一个平行染色体。在这里，我们描述了一个含有视觉调色素基因、转导蛋白α亚单位家族(GNAT)和腺苷环化酶抑制家族(GNAI)、催产素和加压素受体(OT/VP-R)以及L类电压门控钙通道(CACNA1-L)的旁系基因。基于序列的系统发育和保守的同时性分析表明，上述基因家族以及许多邻近的基因家族在脊椎动物早期的WGDS中扩大了。这使得我们可以推断出以下进化情景：脊椎动物的祖先有一条染色体，其中包含两个视觉调控素基因，一个是GNAT，一个是GNAI，两个OT/VP-Rs和一个CACNA1-L基因。这条染色体在2R中翻了四番。随后的基因缺失导致了一组5个视觉视蛋白基因，3个GNAT和GNAI基因，6个OT/VP-R基因和4个CACNA1-L基因。这些区域在3R中再次复制，导致一些家族获得额外的硬骨鱼基因。硬骨鱼基因组中发生了主要的染色体重排。通过与3R之前发散的斑点GAR中相应的染色体区域进行比较，我们可以将这些重排计时到3R后。我们对含有视觉视蛋白、GNAT和GNAI、OT/VP-R和CACNA1-L基因家族的Paralogon进行了广泛的分析。综合数据表明，脊椎动物早期的WGD事件有助于视觉的进化，以及这些基因家族编码的蛋白质所发挥的其他神经和神经内分泌功能。在袋装七鳃鳗中，所有五个视觉视蛋白基因都已被鉴定出来，这表明七鳃鳗在2R之后与有颌骨的脊椎动物发生了分化。
Vertebrate color vision is dependent on four major color opsin subtypes: RH2 (green opsin), SWS1 (ultraviolet opsin), SWS2 (blue opsin), and LWS (red opsin). Together with the dim-light receptor rhodopsin (RH1), these form the family of vertebrate visual opsins. Vertebrate genomes contain many multi-membered gene families that can largely be explained by the two rounds of whole genome duplication (WGD) in the vertebrate ancestor (2R) followed by a third round in the teleost ancestor (3R). Related chromosome regions resulting from WGD or block duplications are said to form a paralogon. We describe here a paralogon containing the genes for visual opsins, the G-protein alpha subunit families for transducin (GNAT) and adenylyl cyclase inhibition (GNAI), the oxytocin and vasopressin receptors (OT/VP-R), and the L-type voltage-gated calcium channels (CACNA1-L). Sequence-based phylogenies and analyses of conserved synteny show that the above-mentioned gene families, and many neighboring gene families, expanded in the early vertebrate WGDs. This allows us to deduce the following evolutionary scenario: The vertebrate ancestor had a chromosome containing the genes for two visual opsins, one GNAT, one GNAI, two OT/VP-Rs and one CACNA1-L gene. This chromosome was quadrupled in 2R. Subsequent gene losses resulted in a set of five visual opsin genes, three GNAT and GNAI genes, six OT/VP-R genes and four CACNA1-L genes. These regions were duplicated again in 3R resulting in additional teleost genes for some of the families. Major chromosomal rearrangements have taken place in the teleost genomes. By comparison with the corresponding chromosomal regions in the spotted gar, which diverged prior to 3R, we could time these rearrangements to post-3R. We present an extensive analysis of the paralogon housing the visual opsin, GNAT and GNAI, OT/VP-R, and CACNA1-L gene families. The combined data imply that the early vertebrate WGD events contributed to the evolution of vision and the other neuronal and neuroendocrine functions exerted by the proteins encoded by these gene families. In pouched lamprey all five visual opsin genes have previously been identified, suggesting that lampreys diverged from the jawed vertebrates after 2R.
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非par体6：具有内核的真核直系同源群。
影响因子：
14.9
作者：
Berglund AC;Sjölund E;Ostlund G;Sonnhammer EL
通讯作者：
Sonnhammer EL
影响因子：
3.4
作者：
Ocampo Daza D;Sundström G;Bergqvist CA;Larhammar D
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影响因子：
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作者：
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通讯作者：
Larhammar, Dan
DOI：
10.1111/j.1095-8312.2007.00864.x
发表时间：
2007-12-01
影响因子：
1.9
作者：
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通讯作者：
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影响因子：
6.5
作者：
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通讯作者：
Gascuel, Olivier