Detection of SARS-CoV-2 in respiratory samples from cats in the UK associated with human-to-cat transmission.
Detection of SARS-CoV-2 in respiratory samples from cats in the UK associated with human-to-cat transmission.
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DOI：
10.1002/vetr.247
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发表时间：
2021-04
期刊：
影响因子：
--
通讯作者：
Weir W
中科院分区：
文献类型：
--
作者：
Hosie MJ;Epifano I;Herder V;Orton RJ;Stevenson A;Johnson N;MacDonald E;Dunbar D;McDonald M;Howie F;Tennant B;Herrity D;Da Silva Filipe A;Streicker DG;COVID-19 Genomics UK (COG-UK) consortium;Willett BJ;Murcia PR;Jarrett RF;Robertson DL;Weir W
目的：本研究的目的是在英国猫中寻找SARS - CoV - 2感染的证据。设计：用免疫荧光法检测组织样本中的SARS - CoV - 2抗原，用原位杂交法检测病毒RNA。采用逆转录酶定量PCR对387份已提交常规呼吸道病原体检测的口咽拭子进行SARS - CoV - 2 RNA检测。结果：1型猫死后采集的肺组织均检测出SARS - CoV - 2核衣壳抗原和RNA阳性。在出现鼻炎和结膜炎的2型患者的口咽拭子中检测到SARS - CoV - 2 RNA。病毒基因组的高通量测序显示，与最近的英国人SARS - CoV - 2序列相比，该病毒有5个单核苷酸多态性（snp），与原始的武汉-胡- 1参考序列相比，该人类病毒含有8个snp。对cat - 2病毒基因组以及来自世界各地的其他9个源自猫的SARS - CoV - 2序列的分析显示，它们没有共同的猫特异性突变。结论：这些发现表明，在英国COVID - 19大流行期间，SARS - CoV - 2发生了人对猫的传播，受感染的猫出现了轻度或重度呼吸道疾病。鉴于新型冠状病毒感染不同物种的能力，监测人对猫、猫对猫和猫对人的传播将非常重要。
Objectives: The aim of the study was to find evidence of SARS‐CoV‐2 infection in UK cats. Design: Tissue samples were tested for SARS‐CoV‐2 antigen using immunofluorescence and for viral RNA by in situ hybridisation. A set of 387 oropharyngeal swabs that had been submitted for routine respiratory pathogen testing was tested for SARS‐CoV‐2 RNA using reverse transcriptase quantitative PCR. Results: Lung tissue collected post‐mortem from cat 1 tested positive for both SARS‐CoV‐2 nucleocapsid antigen and RNA. SARS‐CoV‐2 RNA was detected in an oropharyngeal swab collected from cat 2 that presented with rhinitis and conjunctivitis. High throughput sequencing of the viral genome revealed five single nucleotide polymorphisms (SNPs) compared to the nearest UK human SARS‐CoV‐2 sequence, and this human virus contained eight SNPs compared to the original Wuhan‐Hu‐1 reference sequence. An analysis of the viral genome of cat 2 together with nine other feline‐derived SARS‐CoV‐2 sequences from around the world revealed no shared cat‐specific mutations. Conclusions: These findings indicate that human‐to‐cat transmission of SARS‐CoV‐2 occurred during the COVID‐19 pandemic in the UK, with the infected cats developing mild or severe respiratory disease. Given the ability of the new coronavirus to infect different species, it will be important to monitor for human‐to‐cat, cat‐to‐cat and cat‐to‐human transmission.
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