nanos-Driven expression of piggyBac transposase induces mobilization of a synthetic autonomous transposon in the malaria vector mosquito, Anopheles stephensi.
nanos-Driven expression of piggyBac transposase induces mobilization of a synthetic autonomous transposon in the malaria vector mosquito, Anopheles stephensi.
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DOI：
10.1016/j.ibmb.2017.06.014
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发表时间：
2017-08
影响因子：
3.8
通讯作者：
James AA
中科院分区：
文献类型：
--
作者：
Macias VM;Jimenez AJ;Burini-Kojin B;Pledger D;Jasinskiene N;Phong CH;Chu K;Fazekas A;Martin K;Marinotti O;James AA
转座子是一类可以在基因组内移动的自私DNA元件。如果迁移伴随着拷贝数的增加（复制转座），转座子可以横扫整个种群，直到它在所有杂交成员中固定下来。这种基因渗入被认为是驱动系统将具有理想表型的基因移入目标物种的基础。其中一个应用是利用它们将一种赋予疟疾寄生虫抗性的基因转移到整个媒介蚊子种群中。我们评估了使用piggyBac转座子作为基因驱动机制在疟疾媒介斯氏按蚊种群中分布抗疟疾转基因的可行性。我们设计了合成基因构建体，其使用An的对照DNA在雌性种系中表达piggyBac转座酶。stephensi nanos orthogonal基因与标记基因相连，以监测遗传。观察到两次再动员事件，频率为每23代1次，远低于将抗病原体转基因驱动到野生蚊子种群中的有用速度。我们讨论了优化这一系统的可能性和这样做的动力。
Transposons are a class of selfish DNA elements that can mobilize within a genome. If mobilization is accompanied by an increase in copy number (replicative transposition), the transposon may sweep through a population until it is fixed in all of its interbreeding members. This introgression has been proposed as the basis for drive systems to move genes with desirable phenotypes into target species. One such application would be to use them to move a gene conferring resistance to malaria parasites throughout a population of vector mosquitos. We assessed the feasibility of using the piggyBac transposon as a gene-drive mechanism to distribute anti-malarial transgenes in populations of the malaria vector, Anopheles stephensi. We designed synthetic gene constructs that express the piggyBac transposase in the female germline using the control DNA of the An. stephensi nanos orthologous gene linked to marker genes to monitor inheritance. Two remobilization events were observed with a frequency of one every 23 generations, a rate far below what would be useful to drive anti-pathogen transgenes into wild mosquito populations. We discuss the possibility of optimizing this system and the impetus to do so.
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影响因子：
3.7
作者：
Meredith JM;Underhill A;McArthur CC;Eggleston P
通讯作者：
Eggleston P
影响因子：
4.4
作者：
Fernández-Medina RD;Struchiner CJ;Ribeiro JM
通讯作者：
Ribeiro JM
DOI：
10.1073/pnas.0701515104
发表时间：
2007-06-12
影响因子：
11.1
作者：
Adelman, Zach N.;Jasinskiene, Nijole;James, Anthony A.
通讯作者：
James, Anthony A.
影响因子：
2.4
作者：
Horn, C;Wimmer, EA
通讯作者：
Wimmer, EA
影响因子：
64.5
作者：
Ding, S;Wu, XH;Xu, T
通讯作者：
Xu, T