Conservation of AtTZF1, AtTZF2, and AtTZF3 homolog gene regulation by salt stress in evolutionarily distant plant species.
Conservation of AtTZF1, AtTZF2, and AtTZF3 homolog gene regulation by salt stress in evolutionarily distant plant species.
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DOI：
10.3389/fpls.2015.00394
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发表时间：
2015
影响因子：
5.6
通讯作者：
Mastrangelo AM
中科院分区：
文献类型：
--
作者：
D'Orso F;De Leonardis AM;Salvi S;Gadaleta A;Ruberti I;Cattivelli L;Morelli G;Mastrangelo AM
富含精氨酸的串联锌指蛋白（RR-TZF）参与植物的发育过程和对非生物胁迫（如寒冷、盐和干旱）的适应性反应。本研究探讨了AtTZF 1 -5基因在植物物种间的序列和表达水平上的保守性。从硬粒小麦中分离到两个RR-TZF基因TdTZF 1-A和TdTZF 1-B的基因组序列，并将其分别定位于3A和3B染色体。序列比较显示，它们编码的蛋白质与AtTZF 1、AtTZF 2和AtTZF 3高度同源。这些RR-TZF硬质小麦和拟南芥蛋白的表达谱支持在调节种子萌发和响应非生物胁迫中的共同功能。具体地，对具有减毒和过表达AtTZF 3的植物的分析表明，AtTZF 3是盐胁迫条件下种子萌发的负调节剂。最后，比较序列分析确定RR-TZF基因是由低等植物编码的，包括小立碗藓和莱茵衣藻。盐胁迫对立碗藓AtTZF 1 -2-3样基因的调控强烈表明RR-TZF蛋白的一个亚组具有在整个进化过程中保守的功能。
Arginine-rich tandem zinc-finger proteins (RR-TZF) participate in a wide range of plant developmental processes and adaptive responses to abiotic stress, such as cold, salt, and drought. This study investigates the conservation of the genes AtTZF1-5 at the level of their sequences and expression across plant species. The genomic sequences of the two RR-TZF genes TdTZF1-A and TdTZF1-B were isolated in durum wheat and assigned to chromosomes 3A and 3B, respectively. Sequence comparisons revealed that they encode proteins that are highly homologous to AtTZF1, AtTZF2, and AtTZF3. The expression profiles of these RR-TZF durum wheat and Arabidopsis proteins support a common function in the regulation of seed germination and responses to abiotic stress. In particular, analysis of plants with attenuated and overexpressed AtTZF3 indicate that AtTZF3 is a negative regulator of seed germination under conditions of salt stress. Finally, comparative sequence analyses establish that the RR-TZF genes are encoded by lower plants, including the bryophyte Physcomitrella patens and the alga Chlamydomonas reinhardtii. The regulation of the Physcomitrella AtTZF1-2-3-like genes by salt stress strongly suggests that a subgroup of the RR-TZF proteins has a function that has been conserved throughout evolution.
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影响因子：
11.6
作者：
Li, ZS;Thomas, TL
通讯作者：
Thomas, TL
影响因子：
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作者：
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通讯作者：
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