Self-assembled magnetic filter for highly efficient immunomagnetic separation.
Self-assembled magnetic filter for highly efficient immunomagnetic separation.
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DOI：
10.1039/c0lc00149j
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发表时间：
2011-01-07
期刊：
影响因子：
6.1
通讯作者：
Lee H
中科院分区：
文献类型：
--
作者：
Issadore D;Shao H;Chung J;Newton A;Pittet M;Weissleder R;Lee H
我们已经开发出一种紧凑且廉价的微流控芯片，自组装磁性过滤器，以有效地从悬浮液中去除磁性标记的细胞。自组装磁性过滤器由直接构建在自组装NdFeB磁体上方的微流体通道组成。微米尺寸的NdFe B晶粒聚集以形成交替的磁偶极子，从而在微流体通道中产生具有非常强的幅度B（来自材料）和场梯度B（来自配置）的磁场。施加在磁珠上的磁力被测量为与最先进的微制造磁体相当，从而允许在紧凑、简单的装置中进行有效的分离。磁性过滤器的效率通过将非磁性（聚苯乙烯）珠粒与磁性珠粒（氧化铁）分选来表征。该过滤器将非磁性珠粒的群体富集为磁性珠粒，富集倍数为>105，在1 mL/hr时回收率为90%。磁性过滤器的实用性用微流体装置证明，该微流体装置使用负免疫磁性选择将肿瘤细胞与白细胞分类，并将肿瘤细胞浓缩在集成膜过滤器上用于光学检测。
We have developed a compact and inexpensive microfluidic chip, the Self Assembled Magnetic filter, to efficiently remove magnetically tagged cells from suspension. The self-assembled magnetic filter consists of a microfluidic channel built directly above a self-assembled NdFeB magnet. Micrometer-sized grains of NdFeB assemble to form alternating magnetic dipoles, creating a magnetic field with a very strong magnitude B (from the material) and field gradient ∇B (from the configuration) in the microfluidic channel. The magnetic force imparted on magnetic beads is measured to be comparable to state-of-the-art microfabricated magnets, allowing for efficient separations to be performed in a compact, simple device. The efficiency of the magnetic filter is characterized by sorting non-magnetic (polystyrene) beads from magnetic beads (iron oxide). The filter enriches the population of non-magnetic beads to magnetic beads by a factor of >105 with a recovery rate of 90% at 1 mL/hr. The utility of the magnetic filter is demonstrated with a microfluidic device that sorts tumor cells from leukocytes using negative immunomagnetic selection, and concentrates the tumor cells on an integrated membrane filter for optical detection.
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影响因子：
158.5
作者：
Cristofanilli, M;Budd, GT;Hayes, DF
通讯作者：
Hayes, DF
影响因子：
6.1
作者：
Kim, Unyoung;Soh, H. Tom
通讯作者：
Soh, H. Tom
影响因子：
8.6
作者：
Vieira G;Henighan T;Chen A;Hauser AJ;Yang FY;Chalmers JJ;Sooryakumar R
通讯作者：
Sooryakumar R
影响因子：
3.2
作者：
CROAT, JJ;HERBST, JF;PINKERTON, FE
通讯作者：
PINKERTON, FE
影响因子：
64.8
作者：
MELVILLE, D;PAUL, F;ROATH, S
通讯作者：
ROATH, S