Mechanical forces drive a reorientation cascade leading to biofilm self-patterning.
Mechanical forces drive a reorientation cascade leading to biofilm self-patterning.
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DOI：
10.1038/s41467-021-26869-6
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发表时间：
2021-11-17
影响因子：
16.6
通讯作者：
Yan J
中科院分区：
文献类型：
--
作者：
Nijjer J;Li C;Zhang Q;Lu H;Zhang S;Yan J
在不断增长的活性物质系统中，大量工程或活性自主单元在集体增殖和迁移时代谢自由能并在不同长度尺度上创造秩序。其中一个例子是细菌生物膜，即嵌入细胞外基质中的表面附着的细菌细胞聚集体，可以表现出群落规模的定向顺序。然而，细菌生长如何与细胞表面相互作用协调以在生物膜发育过程中创造独特的长期秩序仍然难以捉摸。在这里，我们报告了生长中的霍乱弧菌生物膜中的集体细胞重新定向级联，该级联导致了差异有序、时空耦合的核心-边缘结构，让人想起盛开的紫苑。核心中的细胞垂直化导致差异生长模式，驱动边缘中的细胞径向排列，而生长的边缘产生压应力，使垂直核心扩张。这种自我模式在非贴壁突变体中消失，但可以通过生长的光操纵来恢复。基于代理的模拟和两相主动向列建模共同揭示了微分排序背后驱动力的强烈相互依赖性。我们的研究结果提供了对塑造细菌群落的发育过程的深入了解，并提供了设计活体活性物质表型和功能的方法。细菌生物膜表现出复杂的时空模式形成。在这里，作者报告了生长中的霍乱弧菌生物膜中的集体细胞重新定向级联，导致了差异有序、时空耦合的核心-边缘结构。
In growing active matter systems, a large collection of engineered or living autonomous units metabolize free energy and create order at different length scales as they proliferate and migrate collectively. One such example is bacterial biofilms, surface-attached aggregates of bacterial cells embedded in an extracellular matrix that can exhibit community-scale orientational order. However, how bacterial growth coordinates with cell-surface interactions to create distinctive, long-range order during biofilm development remains elusive. Here we report a collective cell reorientation cascade in growing Vibrio cholerae biofilms that leads to a differentially ordered, spatiotemporally coupled core-rim structure reminiscent of a blooming aster. Cell verticalization in the core leads to a pattern of differential growth that drives radial alignment of the cells in the rim, while the growing rim generates compressive stresses that expand the verticalized core. Such self-patterning disappears in nonadherent mutants but can be restored through opto-manipulation of growth. Agent-based simulations and two-phase active nematic modeling jointly reveal the strong interdependence of the driving forces underlying the differential ordering. Our findings offer insight into the developmental processes that shape bacterial communities and provide ways to engineer phenotypes and functions in living active matter. Bacterial biofilms exhibit complex spatiotemporal pattern formation. Here the authors report a collective cell reorientation cascade in growing Vibrio cholerae biofilms that leads to a differentially ordered, spatiotemporally coupled core-rim structure.
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DOI：
10.1073/pnas.1212429109
发表时间：
2012-11-13
影响因子：
11.1
作者：
Asally, Munehiro;Kittisopikul, Mark;Sueel, Guerol M.
通讯作者：
Sueel, Guerol M.
DOI：
10.1126/science.1222981
发表时间：
2012-07-13
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Berk V;Fong JC;Dempsey GT;Develioglu ON;Zhuang X;Liphardt J;Yildiz FH;Chu S
通讯作者：
Chu S
DOI：
10.1073/pnas.1504948112
发表时间：
2015-04-28
影响因子：
11.1
作者：
Ghosh, Pushpita;Mondal, Jagannath;Levine, Herbert
通讯作者：
Levine, Herbert
影响因子：
8.6
作者：
Doostmohammadi, Amin;Thampi, Sumesh P.;Yeomans, Julia M.
通讯作者：
Yeomans, Julia M.
影响因子：
6.7
作者：
Absalon C;Van Dellen K;Watnick PI
通讯作者：
Watnick PI