Construction of a SSR-based genetic map and identification of QTLs for catechins content in tea plant (Camellia sinensis).
Construction of a SSR-based genetic map and identification of QTLs for catechins content in tea plant (Camellia sinensis).
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基于SSR的茶树儿茶素遗传图谱构建及QTL鉴定
DOI：
10.1371/journal.pone.0093131
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Chen L
中科院分区：
文献类型：
--
作者：
Ma JQ;Yao MZ;Ma CL;Wang XC;Jin JQ;Wang XM;Chen L
儿茶素是茶叶中最重要的生物活性物质，已被证明具有广泛的药理活性。为了研究茶树嫩梢中儿茶素含量的数量性状基因座(QTL)，利用406个简单序列重复(SSR)标记构建了一个中度饱和遗传图谱，该图谱是基于两个茶树品种不同儿茶素组成的种内杂交183个单株组成的伪测交群体。该图谱由15个连锁群组成，与茶树单倍体染色体数目(2n = 2x = 30)相对应。图谱总长度为1143.5 cM，平均基因座间距为2.9 cM。在两个测量年中，总共确定了25个与儿茶素含量有关的QTL。在这些QTL中，9个稳定的QTL被历年验证，并聚集在LG03、LG11、LG12和LG15上的四个主要染色体区域。各QTL所解释的群体变异以中高水平为主，变幅在2.4%~71.0%之间，平均为17.7%。每个性状的QTL总数变化在4~8个之间，而一个性状的所有QTL解释的总群体变异性在38.4%~79.7%之间。这是首次发现茶树中儿茶素含量的QTL。本研究为儿茶素QTL的进一步克隆和功能鉴定奠定了基础，为茶树改良利用儿茶素QTL奠定了基础。
Catechins are the most important bioactive compounds in tea, and have been demonstrated to possess a wide variety of pharmacological activities. To characterize quantitative trait loci (QTLs) for catechins content in the tender shoots of tea plant, we constructed a moderately saturated genetic map using 406 simple sequence repeat (SSR) markers, based on a pseudo-testcross population of 183 individuals derived from an intraspecific cross of two Camellia sinensis varieties with diverse catechins composition. The map consisted of fifteen linkage groups (LGs), corresponding to the haploid chromosome number of tea plant (2n = 2x = 30). The total map length was 1,143.5 cM, with an average locus spacing of 2.9 cM. A total of 25 QTLs associated with catechins content were identified over two measurement years. Of these, nine stable QTLs were validated across years, and clustered into four main chromosome regions on LG03, LG11, LG12 and LG15. The population variability explained by each QTL was predominantly at moderate-to-high levels and ranged from 2.4% to 71.0%, with an average of 17.7%. The total number of QTL for each trait varied from four to eight, while the total population variability explained by all QTLs for a trait ranged between 38.4% and 79.7%. This is the first report on the identification of QTL for catechins content in tea plant. The results of this study provide a foundation for further cloning and functional characterization of catechin QTLs for utilization in improvement of tea plant.
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