A RAD tag derived marker based eggplant linkage map and the location of QTLs determining anthocyanin pigmentation.
A RAD tag derived marker based eggplant linkage map and the location of QTLs determining anthocyanin pigmentation.
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DOI：
10.1371/journal.pone.0043740
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Rotino GL
中科院分区：
文献类型：
--
作者：
Barchi L;Lanteri S;Portis E;Valè G;Volante A;Pulcini L;Ciriaci T;Acciarri N;Barbierato V;Toppino L;Rotino GL
茄子(Solanum melongena L.)种间和种内图谱均已建立。前者受益于标记多态频率的提高，但它们与标记辅助作物育种的相关性有限。将限制酶切位点相关DNA策略与高通量测序相结合，有助于发现大量区分茄子作图群体亲本‘305E40’和‘67/3’的功能性单核苷酸多态(SNP)标记。将347个SNPs与84个锚定标记一起应用于杂交组合‘305E40’x‘67/3’的F2作图群体，构建了一个连锁图谱。总共，431个标记中的415个被组装成12个主连锁群和1个小连锁群，跨度为1,390 cM，包括已建立的标记，每个连锁群被分配到12条茄子染色体中的一条。然后，该图谱被用来发现与花青素含量相关的七个性状的遗传基础。这些性状均受1~6个数量性状座位(QTL)控制，其中至少有1个是主效QTL。利用茄子和番茄基因组之间的共线关系，有助于确定与花青素积累相关的茄子QTL的潜在候选基因。种内连锁图谱对阐明茄子其他表型性状的遗传基础具有一定的参考价值。
Both inter- and intra-specific maps have been developed in eggplant (Solanum melongena L.). The former benefit from an enhanced frequency of marker polymorphism, but their relevance to marker-assisted crop breeding is limited. Combining the restriction-site associated DNA strategy with high throughput sequencing has facilitated the discovery of a large number of functional single nucleotide polymorphism (SNP) markers discriminating between the two eggplant mapping population parental lines ‘305E40’ and ‘67/3’. A set of 347 de novo SNPs, together with 84 anchoring markers, were applied to the F2 mapping population bred from the cross ‘305E40’ x ‘67/3’ to construct a linkage map. In all, 415 of the 431 markers were assembled into twelve major and one minor linkage group, spanning 1,390 cM, and the inclusion of established markers allowed each linkage group to be assigned to one of the 12 eggplant chromosomes. The map was then used to discover the genetic basis of seven traits associated with anthocyanin content. Each of the traits proved to be controlled by between one and six quantitative trait loci (QTL), of which at least one was a major QTL. Exploitation of syntenic relationships between the eggplant and tomato genomes facilitated the identification of potential candidate genes for the eggplant QTLs related to anthocyanin accumulation. The intra-specific linkage map should have utility for elucidating the genetic basis of other phenotypic traits in eggplant.
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影响因子：
3.1
作者：
Barchi, Lorenzo;Lanteri, Sergio;Rotino, Giuseppe Leonardo
通讯作者：
Rotino, Giuseppe Leonardo
影响因子：
5.4
作者：
De Jong, WS;Eannetta, NT;Bodis, M
通讯作者：
Bodis, M
影响因子：
5.4
作者：
Borovsky, Y;Oren-Shamir, M;Paran, I
通讯作者：
Paran, I
影响因子：
5.8
作者：
Broman, KW;Wu, H;Churchill, GA
通讯作者：
Churchill, GA
影响因子：
5.4
作者：
Li, Z;Jakkula, L;Boerma, HR
通讯作者：
Boerma, HR