Autophagy modulates the metabolism and growth of tomato fruit during development.
Autophagy modulates the metabolism and growth of tomato fruit during development.
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DOI：
10.1093/hr/uhac129
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发表时间：
2022
影响因子：
8.7
通讯作者：
中科院分区：
文献类型：
--
作者：
尽管自噬是真核生物中的一种保守机制，但其对作物尤其是其代谢的影响相对较少受到关注。事实上，虽然最近的一些研究使用系统生物学工具来研究玉米中缺乏自噬的后果，但这些研究重点关注叶组织而不是籽粒。在这里，我们利用 RNA 干扰 (RNAi) 来培育缺乏自噬调节蛋白酶 ATG4 的番茄植株。植物表现出早期衰老表型，但叶代谢组的变化相对轻微，并且以果实产量表型降低为特征。代谢物分析表明，与 WT 相比，ATG4-RNAi 番茄叶片的代谢物仅表现出较小的变化，而果实的代谢物则表现出较大的差异。具体来说，许多初级代谢物在 ATG4-RNAi 系中表现出减少，例如脯氨酸、色氨酸和苯丙氨酸，而代表性的次级代谢物（奎尼酸和 3-反式咖啡酰奎尼酸）在 ATG4-RNAi 绿色果实中的含量明显高于 WT。此外，转录组分析表明，最显着的差异是参与蛋白酶体或叶绿体囊泡途径的细胞器降解基因的显着上调，蛋白质组数据中叶绿体蛋白水平的降低进一步证实了这一点。此外，代谢组、转录组和蛋白质组数据的整合分析表明，ATG4 显着影响脂质代谢、叶绿素结合蛋白和叶绿体生物合成。这些数据共同引导我们提出一个更复杂的模型来解释自噬过程的细胞协调。
Although autophagy is a conserved mechanism operating across eukaryotes, its effects on crops and especially their metabolism has received relatively little attention. Indeed, whilst a few recent studies have used systems biology tools to look at the consequences of lack of autophagy in maize these focused on leaf tissues rather than the kernels. Here we utilized RNA interference (RNAi) to generate tomato plants that were deficient in the autophagy-regulating protease ATG4. Plants displayed an early senescence phenotype yet relatively mild changes in the foliar metabolome and were characterized by a reduced fruit yield phenotype. Metabolite profiling indicated that metabolites of ATG4-RNAi tomato leaves just exhibited minor alterations while that of fruit displayed bigger difference compared to the WT. In detail, many primary metabolites exhibited decreases in the ATG4-RNAi lines, such as proline, tryptophan and phenylalanine, while the representative secondary metabolites (quinic acid and 3-trans-caffeoylquinic acid) were present at substantially higher levels in ATG4-RNAi green fruits than in WT. Moreover, transcriptome analysis indicated that the most prominent differences were in the significant upregulation of organelle degradation genes involved in the proteasome or chloroplast vesiculation pathways, which was further confirmed by the reduced levels of chloroplastic proteins in the proteomics data. Furthermore, integration analysis of the metabolome, transcriptome and proteome data indicated that ATG4 significantly affected the lipid metabolism, chlorophyll binding proteins and chloroplast biosynthesis. These data collectively lead us to propose a more sophisticated model to explain the cellular co-ordination of the process of autophagy.
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影响因子：
7.7
作者：
Dagdas YF;Pandey P;Tumtas Y;Sanguankiattichai N;Belhaj K;Duggan C;Leary AY;Segretin ME;Contreras MP;Savage Z;Khandare VS;Kamoun S;Bozkurt TO
通讯作者：
Bozkurt TO
影响因子：
7.4
作者：
Lytovchenko, Anna;Eickmeier, Ira;Fernie, Alisdair R.
通讯作者：
Fernie, Alisdair R.
影响因子：
5.9
作者：
Luzarowski M;Vicente R;Kiselev A;Wagner M;Schlossarek D;Erban A;de Souza LP;Childs D;Wojciechowska I;Luzarowska U;Górka M;Sokołowska EM;Kosmacz M;Moreno JC;Brzezińska A;Vegesna B;Kopka J;Fernie AR;Willmitzer L;Ewald JC;Skirycz A
通讯作者：
Skirycz A
影响因子：
18
作者：
McLoughlin, Fionn;Augustine, Robert C.;Vierstra, Richard D.
通讯作者：
Vierstra, Richard D.
影响因子：
11.6
作者：
Masclaux-Daubresse, Celine;Clement, Gilles;Yoshimoto, Kohki
通讯作者：
Yoshimoto, Kohki