Functional remineralization of dentin lesions using polymer-induced liquid-precursor process.
Functional remineralization of dentin lesions using polymer-induced liquid-precursor process.
复制标题
DOI：
10.1371/journal.pone.0038852
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Marshall GW
中科院分区：
文献类型：
--
作者：
Burwell AK;Thula-Mata T;Gower LB;Habelitz S;Kurylo M;Ho SP;Chien YC;Cheng J;Cheng NF;Gansky SA;Marshall SJ;Marshall GW
据推测，将聚合物诱导的液体前体（PILP）系统应用于人工病变将导致龋性牙本质病变的时间依赖性功能性再矿化，恢复脱矿牙本质基质的机械性能。通过PILP过程在37°C下使140 µm深的人工龋损伤再矿化7-28天，以确定时间再矿化特征。聚-L-天冬氨酸（27 KDa）用作聚合过程导向剂，并以2.14（mol/mol）的钙与磷酸盐比率添加到矿化溶液中。水合人工损伤的纳米力学性质具有延伸到损伤中约70 μm的低降低弹性模量（ER=0.2GPa）区域，具有延伸到约140 μm的倾斜区域，其中值达到正常牙本质（18-20 GPa）。 7天后，标本恢复机械性能的斜坡区相比，人工病变的51%。在7-14天之间，病变外部的恢复持续到约10 GPa的水平，改善74%。与人工损伤相比，PILP矿化28天导致ER改善91%。这些差异具有统计学显著性，如从变化点图中确定的。通过微型X射线计算机断层扫描确定的矿物分布比通过纳米压痕确定的矿物分布浅，并且随着时间的推移显示出类似的变化，但是在病变的外部和倾斜部分中，14天后发生了完全的矿物恢复。扫描电子显微镜和能量色散X射线分析显示，与正常牙本质不同的是一个明确的分界线之间的外部和倾斜的部分病变的相似的形态。透射电子显微镜和选区电子衍射表明，起始病变包含一些残留的矿物在外部部分，表现出较差的结晶度。在牙本质矿化过程中，原纤维内矿物质增加，结晶度提高，原纤维内矿物质表现出正常牙本质或骨中发现的取向。
It was hypothesized that applying the polymer-induced liquid-precursor (PILP) system to artificial lesions would result in time-dependent functional remineralization of carious dentin lesions that restores the mechanical properties of demineralized dentin matrix. 140 µm deep artificial caries lesions were remineralized via the PILP process for 7–28 days at 37°C to determine temporal remineralization characteristics. Poly-L-aspartic acid (27 KDa) was used as the polymeric process-directing agent and was added to the remineralization solution at a calcium-to-phosphate ratio of 2.14 (mol/mol). Nanomechanical properties of hydrated artificial lesions had a low reduced elastic modulus (ER = 0.2 GPa) region extending about 70 μm into the lesion, with a sloped region to about 140 μm where values reached normal dentin (18–20 GPa). After 7 days specimens recovered mechanical properties in the sloped region by 51% compared to the artificial lesion. Between 7–14 days, recovery of the outer portion of the lesion continued to a level of about 10 GPa with 74% improvement. 28 days of PILP mineralization resulted in 91% improvement of ER compared to the artificial lesion. These differences were statistically significant as determined from change-point diagrams. Mineral profiles determined by micro x-ray computed tomography were shallower than those determined by nanoindentation, and showed similar changes over time, but full mineral recovery occurred after 14 days in both the outer and sloped portions of the lesion. Scanning electron microscopy and energy dispersive x-ray analysis showed similar morphologies that were distinct from normal dentin with a clear line of demarcation between the outer and sloped portions of the lesion. Transmission electron microscopy and selected area electron diffraction showed that the starting lesions contained some residual mineral in the outer portions, which exhibited poor crystallinity. During remineralization, intrafibrillar mineral increased and crystallinity improved with intrafibrillar mineral exhibiting the orientation found in normal dentin or bone.
登录
查看更多内容
影响因子：
7.6
作者：
Sulkala, M;Wahlgren, J;Tjäderhane, L
通讯作者：
Tjäderhane, L
在羟基磷灰石成核抑制剂存在下胶原蛋白在骨磷灰石形成中的作用。
影响因子：
41.2
作者：
通讯作者：
--
影响因子：
9.7
作者：
Kim, Jongryul;Arola, Dwayne D.;Gu, Lisha;Kim, Young Kyung;Mai, Sui;Liu, Yan;Pashley, David H.;Tay, Franklin R.
通讯作者：
Tay, Franklin R.
影响因子：
14
作者：
Kim YK;Gu LS;Bryan TE;Kim JR;Chen L;Liu Y;Yoon JC;Breschi L;Pashley DH;Tay FR
通讯作者：
Tay FR
影响因子：
62.1
作者：
Gower, Laurie B.
通讯作者：
Gower, Laurie B.