Transcriptome analysis of chicken kidney tissues following coronavirus avian infectious bronchitis virus infection.
Transcriptome analysis of chicken kidney tissues following coronavirus avian infectious bronchitis virus infection.
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DOI：
10.1186/1471-2164-14-743
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发表时间：
2013-10-30
期刊：
影响因子：
4.4
通讯作者：
Liu S
中科院分区：
文献类型：
--
作者：
Cong F;Liu X;Han Z;Shao Y;Kong X;Liu S
传染性支气管炎病毒（IBV）是冠状病毒科的原型，是鸡传染性支气管炎的经济上重要的病原体，并且引起急性和高度接触性的上呼吸道感染，其可导致肾炎。然而，鸡对IBV感染的分子抗病毒机制仍然知之甚少。为了更好地了解IBV感染后鸡肾组织的基因表达谱变化，本研究对IBV感染后鸡肾组织的基因表达谱进行了研究。与对照鸡相比，IBV感染导致1777个基因表达差异，其中876个基因表达上调，901个基因表达下调，103个与免疫和炎症反应相关的基因可能在IBV感染时的宿主防御反应中起重要作用。12个改变的免疫相关基因通过实时RT-PCR证实。分析基因本体分类、KEGG通路和基因相互作用网络（STRING分析）以确定参与信号转导、细胞粘附、免疫应答、凋亡调节、I-kappaB激酶/NF-κ B级联的正调节和对细胞因子刺激的应答的差异表达基因之间的关系。这些基因大多相互关联，形成了一个大的网络，其中IL 6、STAT 1、MYD 88、IRF 1和NFKB 2是关键基因。我们的研究结果提供了关于鸡肾组织中宿主对IBV感染的转录反应的全面知识，从而提供了对IBV发病机制的深入了解，特别是与IBV感染相关的先天免疫途径基因的参与。
Infectious bronchitis virus (IBV), a prototype of the Coronaviridae family, is an economically important causative agent of infectious bronchitis in chickens and causes an acute and highly contagious upper respiratory tract infections that may lead to nephritis. However, the molecular antiviral mechanisms of chickens to IBV infection remain poorly understood. In this study, we conducted global gene expression profiling of chicken kidney tissue after nephropathogenic IBV infection to better understand the interactions between host and virus. IBV infection contributed to differential expression of 1777 genes, of which 876 were up-regulated and 901 down-regulated in the kidney compared to those of control chickens and 103 associated with immune and inflammatory responses may play important roles in the host defense response during IBV infection. Twelve of the altered immune-related genes were confirmed by real-time RT-PCR. Gene ontology category, KEGG pathway, and gene interaction networks (STRING analysis) were analyzed to identify relationships among differentially expressed genes involved in signal transduction, cell adhesion, immune responses, apoptosis regulation, positive regulation of the I-kappaB kinase/NF-kappaB cascade and response to cytokine stimulus. Most of these genes were related and formed a large network, in which IL6, STAT1, MYD88, IRF1 and NFKB2 were key genes. Our results provided comprehensive knowledge regarding the host transcriptional response to IBV infection in chicken kidney tissues, thereby providing insight into IBV pathogenesis, particularly the involvement of innate immune pathway genes associated with IBV infection.
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影响因子：
3.3
作者：
Liu X;Ma H;Xu Q;Sun N;Han Z;Sun C;Guo H;Shao Y;Kong X;Liu S
通讯作者：
Liu S
影响因子：
1.4
作者：
KINDE, H;DAFT, BM;REYNOLDS, B
通讯作者：
REYNOLDS, B
影响因子：
3.7
作者：
Danesh A;Cameron CM;León AJ;Ran L;Xu L;Fang Y;Kelvin AA;Rowe T;Chen H;Guan Y;Jonsson CB;Cameron MJ;Kelvin DJ
通讯作者：
Kelvin DJ
影响因子：
4.3
作者：
Jones, R. M.;Ellis, R. J.;Wakeley, P. R.
通讯作者：
Wakeley, P. R.
影响因子：
3.9
作者：
Haller, Otto;Staeheli, Peter;Kochs, Georg
通讯作者：
Kochs, Georg