Accumulation of fibronectin in the heart after myocardial infarction: a putative stimulator of adhesion and proliferation of adipose-derived stem cells.
Accumulation of fibronectin in the heart after myocardial infarction: a putative stimulator of adhesion and proliferation of adipose-derived stem cells.
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DOI：
10.1007/s00441-008-0573-0
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发表时间：
2008-05
影响因子：
3.6
通讯作者：
van Milligen, F. J.
中科院分区：
文献类型：
--
作者：
van Dijk, A.;Niessen, H. W. M.;Ursem, W.;Twisk, J. W. R.;Visser, F. C.;van Milligen, F. J.
干细胞治疗是一种很有前途的治疗心肌梗死的方法。然而，干细胞治疗的一个主要问题是，只有一小部分用于心脏的干细胞能够存活并分化为心肌细胞。我们假设心肌梗死后心脏中的纤维连接蛋白可能对损伤部位的干细胞粘附和增殖产生积极影响。因此，我们研究了脂肪组织源性干细胞（ASC）在纤连蛋白上的附着和增殖动力学，并分析了心肌梗死后纤连蛋白在人心脏中积累的时间框架和位置。将ASC植入纤连蛋白包被和未包被的培养孔中。不同孵育时间（5 - 30min）后，采用DNA定量法测定黏附ASC的数量。培养不同时期（0 ~ 9天）后，采用DNA法定量测定ASC的增殖情况。采用免疫组化染色法对35例患者不同梗死期（0 ~ 14 d）心肌切片进行心肌梗死后纤维连接蛋白积累定量。我们发现，在纤维连接蛋白包被的培养孔中，ASC的附着和增殖明显高于未包被的培养孔。与未梗死心脏相比，梗死后12小时至14天，梗死区和边缘区纤维连接蛋白沉积均显著增加。我们的研究结果表明，纤连蛋白对心脏干细胞的积极作用只有在心肌梗死后至少12小时进行干细胞治疗时才能实现，此时纤连蛋白在梗死心脏中积累。
Stem cell therapy is a promising treatment after myocardial infarction (MI). A major problem in stem cell therapy, however, is that only a small proportion of stem cells applied to the heart can survive and differentiate into cardiomyocytes. We hypothesized that fibronectin in the heart after MI might positively affect stem cell adhesion and proliferation at the site of injury. Therefore, we investigated the kinetics of attachment and proliferation of adipose-tissue-derived stem cells (ASC) on fibronectin and analysed the time frame and localization of fibronectin accumulation in the human heart after MI. ASCs were seeded onto fibronectin-coated and uncoated culture wells. The numbers of adhering ASC were quantified after various incubation periods (5–30 min) by using DNA quantification assays. The proliferation of ASC was quantified after culturing ASC for various periods (0–9 days) by using DNA assays. Fibronectin accumulation after MI was quantified by immunohistochemical staining of heart sections from 35 patients, after different infarction periods (0–14 days old). We found that ASC attachment and proliferation on fibronectin-coated culture wells was significantly higher than on uncoated wells. Fibronectin deposition was significantly increased from 12 h to 14 days post-infarction, both in the infarction area and in the border-zone, compared with the uninfarcted heart. Our results suggest that a positive effect of fibronectin on stem cells in the heart can only be achieved when stem cell therapy is applied at least 12 h after MI, when the accumulation of fibronectin occurs in the infarcted heart.
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影响因子：
--
作者：
Salasznyk RM;Williams WA;Boskey A;Batorsky A;Plopper GE
通讯作者：
Plopper GE
DOI：
10.1253/jcj.60.108
发表时间：
1996-02-01
期刊：
JAPANESE CIRCULATION JOURNAL-ENGLISH EDITION
影响因子：
--
作者：
Morishita, N;Kusachi, S;Tsuji, T
通讯作者：
Tsuji, T
影响因子：
9.5
作者：
Ma, J;Ge, JB;Zou, YZ
通讯作者：
Zou, YZ
影响因子：
5
作者：
SHEKHONIN, BV;GURIEV, SB;KOTELIANSKY, VE
通讯作者：
KOTELIANSKY, VE
影响因子：
4.5
作者：
Oedayrajsingh-Varma, MJ;van Ham, SM;van Milligen, FJ
通讯作者：
van Milligen, FJ