Regulation of reactive oxygen and nitrogen species by salicylic acid in rice plants under salinity stress conditions.
Regulation of reactive oxygen and nitrogen species by salicylic acid in rice plants under salinity stress conditions.
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DOI：
10.1371/journal.pone.0192650
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Lee IJ
中科院分区：
文献类型：
--
作者：
Kim Y;Mun BG;Khan AL;Waqas M;Kim HH;Shahzad R;Imran M;Yun BW;Lee IJ
研究了外源水杨酸（SA）对水稻短期盐胁迫下活性氧和氮的调控作用。SA应用程序（0.5和1.0 mM）在盐诱导的应力（100 mM NaCl）导致显着较长的芽长和较高的叶绿素和生物量积累比单独的盐胁迫。NaCl诱导的活性氧产生导致水稻植株中脂质过氧化水平的增加，SA施用后显著降低。一个类似的发现，观察超氧化物歧化酶，但是，过氧化氢酶（CAT）和抗坏血酸过氧化物酶（APX）显着减少在水稻植株与SA和NaCl单独和组合处理。在SA胁迫下，水稻植株中OsCATA和OsAPX 1的相对mRNA表达量较低。关于含氮物质，S-亚硝基硫醇（SNO）显着减少最初（治疗后一天[DAT]），但随后增加在植物中进行单一或组合的应力条件。还评估了与SNO生物合成、S-亚硝基谷胱甘肽还原酶（GSNOR 1）、NO还原酶样活性（NOA）和亚硝酸盐还原酶（NIR）相关的基因。GSNOR 1的mRNA表达相对于对照增加，而OsNOA在SA和NaCl单独处理的植物中的表达水平相对于对照更高。与对照相比，除2DAT外，单独或组合处理的植物中OsNR的mRNA表达量均降低。总之，目前的研究结果表明，SA可以调节NaCl诱导的氧和氮活性物种在水稻植株中的产生。
This study investigated the regulatory role of exogenous salicylic acid (SA) in rice and its effects on toxic reactive oxygen and nitrogen species during short-term salinity stress. SA application (0.5 and 1.0 mM) during salinity-induced stress (100 mM NaCl) resulted in significantly longer shoot length and higher chlorophyll and biomass accumulation than with salinity stress alone. NaCl-induced reactive oxygen species production led to increased levels of lipid peroxidation in rice plants, which were significantly reduced following SA application. A similar finding was observed for superoxide dismutase; however, catalase (CAT) and ascorbate peroxidase (APX) were significantly reduced in rice plants treated with SA and NaCl alone and in combination. The relative mRNA expression of OsCATA and OsAPX1 was lower in rice plants during SA stress. Regarding nitrogenous species, S-nitrosothiol (SNO) was significantly reduced initially (one day after treatment [DAT]) but then increased in plants subjected to single or combined stress conditions. Genes related to SNO biosynthesis, S-nitrosoglutathione reductase (GSNOR1), NO synthase-like activity (NOA), and nitrite reductase (NIR) were also assessed. The mRNA expression of GSNOR1 was increased relative to that of the control, whereas OsNOA was expressed at higher levels in plants treated with SA and NaCl alone relative to the control. The mRNA expression of OsNR was decreased in plants subjected to single or combination treatment, except at 2 DAT, compared to the control. In conclusion, the current findings suggest that SA can regulate the generation of NaCl-induced oxygen and nitrogen reactive species in rice plants.
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