LecRK-V, an L-type lectin receptor kinase in Haynaldia villosa, plays positive role in resistance to wheat powdery mildew.
LecRK-V, an L-type lectin receptor kinase in Haynaldia villosa, plays positive role in resistance to wheat powdery mildew.
复制标题
LecRK-V是Haynaldia villosa中的L型凝集素受体激酶，在抗小麦白粉病中发挥积极作用
DOI：
10.1111/pbi.12748
复制
发表时间：
2018-01
影响因子：
13.8
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Wang Z;Cheng J;Fan A;Zhao J;Yu Z;Li Y;Zhang H;Xiao J;Muhammad F;Wang H;Cao A;Xing L;Wang X
植物利用模式识别受体（PRRs）识别病原体相关分子模式（PAMP）来感知潜在的微生物病原体。凝集素受体样激酶基因（LecRKs）参与信号转导途径介导的各种细胞过程。在本研究中，L-型凝集素受体激酶基因LecRK-V克隆簇毛麦，一个二倍体小麦近缘种，是高度抗白粉病。LecRK-V的表达通过Bgt接种和几丁质处理而迅速上调。其在叶片中的转录水平高于根、茎、穗和愈伤组织。LecRK-V的单细胞瞬时过表达导致小麦品种扬麦158吸器指数降低，这是白粉病敏感的。将LecRK-V稳定转化到扬麦158中显著增强了苗期和成株期的白粉病抗性。在苗期，转基因株系对23个Bgt菌株中的18个菌株表现高抗，对22个菌株表现过敏反应（HR），并在Bgt侵染部位积累了较多的活性氧。这些表明LecRK-V赋予对白粉病的广谱抗性，ROS和SA途径有助于增强小麦的白粉病抗性。
Plant sense potential microbial pathogen using pattern recognition receptors (PRRs) to recognize pathogen‐associated molecular patterns (PAMPs). The Lectin receptor‐like kinase genes (LecRKs) are involved in various cellular processes mediated by signal transduction pathways. In the present study, an L‐type lectin receptor kinase gene LecRK‐V was cloned from Haynaldia villosa, a diploid wheat relative which is highly resistant to powdery mildew. The expression of LecRK‐V was rapidly up‐regulated by Bgt inoculation and chitin treatment. Its transcript level was higher in the leaves than in roots, culms, spikes and callus. Single‐cell transient overexpression of LecRK‐V led to decreased haustorium index in wheat variety Yangmai158, which is powdery mildew susceptible. Stable transformation LecRK‐V into Yangmai158 significantly enhanced the powdery mildew resistance at both seedling and adult stages. At seedling stage, the transgenic line was highly resistance to 18 of the tested 23 Bgt isolates, hypersensitive responses (HR) were observed for 22 Bgt isolates, and more ROS at the Bgt infection sites was accumulated. These indicated that LecRK‐V confers broad‐spectrum resistance to powdery mildew, and ROS and SA pathways contribute to the enhanced powdery mildew resistance in wheat.
登录
查看更多内容
DOI：
10.1073/pnas.1016981108
发表时间：
2011-05-10
影响因子：
11.1
作者：
Cao, Aizhong;Xing, Liping;Chen, Peidu
通讯作者：
Chen, Peidu
影响因子：
7.2
作者：
Hurni, Severine;Brunner, Susanne;Keller, Beat
通讯作者：
Keller, Beat
影响因子：
64.8
作者：
通讯作者：
--
DOI：
10.1126/science.1171647
发表时间：
2009-05-08
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Boller T;He SY
通讯作者：
He SY
DOI：
10.1073/pnas.0508882103
发表时间：
2006-07-18
影响因子：
11.1
作者：
Kaku, Hanae;Nishizawa, Yoko;Shibuya, Naoto
通讯作者：
Shibuya, Naoto