The Effect of SkitoSnack, an Artificial Blood Meal Replacement, on Aedes aegypti Life History Traits and Gut Microbiota.
The Effect of SkitoSnack, an Artificial Blood Meal Replacement, on Aedes aegypti Life History Traits and Gut Microbiota.
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DOI：
10.1038/s41598-018-29415-5
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发表时间：
2018-07-23
影响因子：
4.6
通讯作者：
Hansen IA
中科院分区：
文献类型：
--
作者：
Gonzales KK;Rodriguez SD;Chung HN;Kowalski M;Vulcan J;Moore EL;Li Y;Willette SM;Kandel Y;Van Voorhies WA;Holguin FO;Hanley KA;Hansen IA
公共卫生研究和病媒控制经常需要饲养大量病媒蚊子。所有目标媒介蚊子物种都是自体的，这意味着雌性蚊子需要脊椎动物的血液来产卵。然而，脊椎动物的血液价格昂贵，保质期短。为了克服这些限制，我们开发了SkitoSnack，这是一种人工血粉替代品，用于蚊子埃及伊蚊，登革热，寨卡和基孔肯雅病毒的载体。SkitoSnack含有牛血清白蛋白和血红蛋白作为蛋白质来源，以及蛋黄和碳酸氢盐缓冲液。SkitoSnack饲养的雌性动物与血液饲养的雌性动物具有可比的生活史特征。从SkitoSnack喂养的雌性饲养的蚊子在用2型登革病毒（DENV-2）经口攻击时具有相似的感染和传播水平，而DENV-4的感染显著较低。当SkitoSnack用作DENV-2递送的媒介物时，血液饲养的和SkitoSnack饲养的雌性动物同样易感。中肠微生物群在以SkitoSnack喂养的蚊子和以血液喂养的蚊子之间存在显着差异。通过在SkitoSnack上饲养20代伊蚊，我们已经证明这种人工饲料可以取代蚊子大规模饲养中的血液。
Public health research and vector control frequently require the rearing of large numbers of vector mosquitoes. All target vector mosquito species are anautogenous, meaning that females require vertebrate blood for egg production. Vertebrate blood, however, is costly, with a short shelf life. To overcome these constraints, we have developed SkitoSnack, an artificial blood meal replacement for the mosquito Aedes aegypti, the vector of dengue, Zika and chikungunya virus. SkitoSnack contains bovine serum albumin and hemoglobin as protein source as well as egg yolk and a bicarbonate buffer. SkitoSnack-raised females had comparable life history traits as blood-raised females. Mosquitoes reared from SkitoSnack-fed females had similar levels of infection and dissemination when orally challenged with dengue virus type 2 (DENV-2) and significantly lower infection with DENV-4. When SkitoSnack was used as a vehicle for DENV-2 delivery, blood-raised and SkitoSnack-raised females were equally susceptible. The midgut microbiota differed significantly between mosquitoes fed on SkitoSnack and mosquitoes fed on blood. By rearing 20 generations of Aedes exclusively on SkitoSnack, we have proven that this artificial diet can replace blood in mosquito mass rearing.
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影响因子：
3.2
作者：
Abdi, Herve;Williams, Lynne J.
通讯作者：
Williams, Lynne J.
影响因子：
3.8
作者：
Carvalho DO;McKemey AR;Garziera L;Lacroix R;Donnelly CA;Alphey L;Malavasi A;Capurro ML
通讯作者：
Capurro ML
影响因子：
4.6
作者：
Dutra HLC;Rodrigues SL;Mansur SB;de Oliveira SP;Caragata EP;Moreira LA
通讯作者：
Moreira LA
DOI：
10.3390/v3091562
发表时间：
2011-09
期刊：
Viruses
影响因子：
--
作者：
Chen R;Vasilakis N
通讯作者：
Vasilakis N
DOI：
10.1098/rspb.2007.1497
发表时间：
2008-02-22
影响因子：
4.7
作者：
Alto, Barry W.;Lounibos, L. Philip;Reiskind, Michael H.
通讯作者：
Reiskind, Michael H.