The response of dominant and rare taxa for fungal diversity within different root environments to the cultivation of Bt and conventional cotton varieties.
The response of dominant and rare taxa for fungal diversity within different root environments to the cultivation of Bt and conventional cotton varieties.
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不同根系环境中优势和稀有类群真菌多样性对 Bt 和常规棉花品种种植的响应
DOI：
10.1186/s40168-018-0570-9
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发表时间：
2018-10-18
期刊：
影响因子：
15.5
通讯作者：
Ming F
中科院分区：
文献类型：
--
作者：
Li P;Xue Y;Shi J;Pan A;Tang X;Ming F
在过去的几十年里，苏云金芽孢杆菌(Bt)作物被大规模种植，这引起了人们对自然环境意外影响的担忧。微生物群落通常包含许多稀有的类群，它们构成了群落种群的大多数。然而，真菌多样性的优势类群和稀有类群对Bt植物不同根环境的响应尚不清楚。我们通过定量聚合酶链式反应和18S rRNA基因测序，分别对不同生长阶段Bt和常规棉花品种根际土壤中的真菌种群数量和群落组成进行了量化。QPCR分析表明，转Bt基因抗虫棉SGK321的根际真菌丰度在苗期达到高峰，其主根和侧根根际差异显著。然而，相同根区的Bt抗虫棉与常规抗虫棉品种的种群大小并无显著差异。真菌属丰富度的总体格局遵循优势属的格局，真菌属丰富度的总体格局遵循稀有属的格局。这些结果表明，优势类群和稀有类群在维持根际微生境生态系统中起着不同的作用。聚类分析表明，苗期3个棉花品种的侧根或主根真菌群落发生了明显的分化，说明根际微生境对真菌群落组成有显著影响。冗余度分析表明，pH与土壤真菌群落组成的相关性大于Bt蛋白含量。综上所述，这些结果表明，在Bt和常规棉花品种不同的根部微生境中，优势真菌和稀有真菌对群落动态的贡献是不同的。此外，这些结果表明，与两个常规棉花品种相比，转Bt基因棉的根际真菌群落对Bt蛋白的存在没有显著的响应。本文的在线版本(10.1186/s401680180570-9)包含补充材料，可供授权用户使用。
Bacillus thuringiensis (Bt) crops have been cultivated at a large scale over the past several decades, which have raised concern about unintended effects on natural environments. Microbial communities typically contain numerous rare taxa that make up the majority of community populations. However, the response of dominant and rare taxa for fungal diversity to the different root environments of Bt plants remains unclear. We quantified fungal population sizes and community composition via quantitative PCR of ITS genes and 18S rRNA gene sequencing of, respectively, that were associated with Bt and conventional cotton variety rhizosphere soils from different plant growth stages. qPCR analyses indicated that fungal abundances reached their peak at the seedling stage and that the taproots and lateral root rhizospheres of the Bt cotton SGK321 were significantly different. However, no significant differences in population sizes were detected between the same root zones from Bt and the conventional cotton varieties. The overall patterns of fungal genera abundances followed that of the dominant genera, whereas overall patterns of fungal genera richness followed those of the rare genera. These results suggest that the dominant and rare taxa play different roles in the maintenance of rhizosphere microhabitat ecosystems. Cluster analyses indicated a separation of fungal communities based on the lateral roots or taproots from the three cotton varieties at the seedling stage, suggesting that root microhabitats had marked effects on fungal community composition. Redundancy analyses indicated that pH was more correlated to soil fungal community composition than Bt protein content. In conclusion, these results indicate that dominant and rare fungal taxa differentially contribute to community dynamics in different root microhabitats of both Bt and conventional cotton varieties. Moreover, these results showed that the rhizosphere fungal community of Bt cotton did not respond significantly to the presence of Bt protein when compared to the two conventional cotton varieties. The online version of this article (10.1186/s40168-018-0570-9) contains supplementary material, which is available to authorized users.
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影响因子：
5.6
作者：
Koch MS;Ward JM;Levine SL;Baum JA;Vicini JL;Hammond BG
通讯作者：
Hammond BG
影响因子：
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Li P;Ye S;Liu H;Pan A;Ming F;Tang X
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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影响因子：
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