Single Nucleotide Polymorphisms of Immunity-Related Genes and Their Effects on Immunophenotypes in Different Pig Breeds.
Single Nucleotide Polymorphisms of Immunity-Related Genes and Their Effects on Immunophenotypes in Different Pig Breeds.
复制标题
DOI：
10.3390/genes12091377
复制
发表时间：
2021-08-31
期刊：
影响因子：
3.5
通讯作者：
Chaung HC
中科院分区：
文献类型：
--
作者：
Chen AY;Huang CW;Liu SH;Liu AC;Chaung HC
增强对病原体的抗性和耐受性仍然是畜禽生产中的一个重要选择目标。单核苷酸多态性（SNPs）在转录水平上改变基因表达，影响个体的免疫调节和对疾病的易感性。在本研究中，我们研究了纯种猪（台湾黑猪、杜洛克猪、长白猪和约克猪）和杂交猪（长白猪-约克猪）免疫相关基因SNP位点的分布及其与免疫细胞表面标志物的相关性。选取免疫相关基因39个snp，包括11个细胞因子、5个趋化因子和23个toll样受体(干扰素-α和γ (IFN-α, γ)、肿瘤坏死因子-α (TNF-α)、粒细胞-巨噬细胞集落刺激因子（GM-CSF）、单核细胞趋化蛋白-1 （MCP-1）和TLR3、TLR4、TLR7、TLR8、TLR9)，分析CD4、CD8、CD80/86、MHCI、MHCII 5种细胞表面标志物的阳性细胞百分比。品种间有28个snp存在显著差异，尤其是长白与台湾黑。例如，SNP1 IFN-α -235A/G在台湾黑种人和长白种人中的频率分别为11.11%和96.15%。此外，18个snp与细胞表面标志物的表达显著相关，包括CD4、CD8、CD80/86和MHCII。SNP33 TLR-8 543C/C中CD4+的比例（39.27%）显著高于A/C(24.34%)，差异有统计学意义（p < 0.05）。总之，我们的研究结果表明台湾黑猪具有独特的基因型分布，而长白猪和约克郡猪具有更相似的基因型分布。因此，了解每个品种的遗传独特性有助于识别免疫调节中功能重要的snp。
Enhancing resistance and tolerance to pathogens remains an important selection objective in the production of livestock animals. Single nucleotide polymorphisms (SNPs) vary gene expression at the transcriptional level, influencing an individual’s immune regulation and susceptibility to diseases. In this study, we investigated the distribution of SNP sites in immune-related genes and their correlations with cell surface markers of immune cells within purebred (Taiwan black, Duroc, Landrace and Yorkshire) and crossbred (Landrace-Yorkshire) pigs. Thirty-nine SNPs of immune-related genes, including 11 cytokines, 5 chemokines and 23 Toll-like receptors (TLRs) (interferon-α and γ (IFN-α, γ), tumor necrosis factor-α (TNF-α), granulocyte-macrophage colony-stimulating factor (GM-CSF), Monocyte chemoattractant protein-1 (MCP-1) and TLR3, TLR4, TLR7, TLR8, and TLR9) were selected, and the percentages of positive cells with five cell surface markers of CD4, CD8, CD80/86, MHCI, and MHCII were analyzed. There were 28 SNPs that were significantly different among breeds, particularly between Landrace and Taiwan black. For instance, the frequency of SNP1 IFN-α -235A/G in Taiwan black and Landrace was 11.11% and 96.15%, respectively. In addition, 18 SNPs significantly correlated with the expression of cell surface markers, including CD4, CD8, CD80/86, and MHCII. The percentage of CD4+ (39.27%) in SNP33 TLR-8 543C/C was significantly higher than those in A/C (24.34%), at p < 0.05. Together, our findings show that Taiwan black pigs had a unique genotype distribution, whereas Landrace and Yorkshire had a more similar genotype distribution. Thus, an understanding of the genetic uniqueness of each breed could help to identify functionally important SNPs in immunoregulation.
登录
查看更多内容
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
3.7
作者：
Flori L;Gao Y;Laloë D;Lemonnier G;Leplat JJ;Teillaud A;Cossalter AM;Laffitte J;Pinton P;de Vaureix C;Bouffaud M;Mercat MJ;Lefèvre F;Oswald IP;Bidanel JP;Rogel-Gaillard C
通讯作者：
Rogel-Gaillard C
影响因子：
4.4
作者：
Hornell, TMC;Beresford, GW;Mellins, ED
通讯作者：
Mellins, ED
DOI：
10.1073/pnas.0609954104
发表时间：
2007-01-16
影响因子：
11.1
作者：
Huang, Ying;Yang, Huiying;Liang, T. Jake
通讯作者：
Liang, T. Jake
影响因子：
3.2
作者：
Bergman, Ingrid-Maria;Rosengren, Johan K.;Edfors, Inger
通讯作者：
Edfors, Inger