Identification of the chemical constituents in aqueous extract of Zhi-Qiao and evaluation of its antidepressant effect.
Identification of the chemical constituents in aqueous extract of Zhi-Qiao and evaluation of its antidepressant effect.
复制标题
栀翘水提物化学成分的鉴定及其抗抑郁作用评价
DOI：
10.3390/molecules20046925
复制
发表时间：
2015-04-16
期刊：
影响因子：
--
通讯作者：
Zou Z
中科院分区：
文献类型：
--
作者：
Wu M;Zhang H;Zhou C;Jia H;Ma Z;Zou Z
枳实（Citrus aurantium L., Zhi-Qiao， ZQ）的未成熟果实在中国已被用作传统药物。我们前期的研究表明，ZQ汤可能与柴胡疏肝散的抗抑郁作用类似。然而，目前还没有关于ZQ水提物的化学成分和抗抑郁作用的报道。首先，采用超高效液相色谱/飞行时间质谱联用技术（UPLC-Q-TOF/MS）在线鉴定了ZQ水提物中的化学成分。共鉴定出31种化学成分，包括1种单宁酸、5种黄酮、13种黄酮、1种柠檬素、3种香豆素、3种环肽和5种多甲氧基黄酮类化合物。实验结果表明，ZQ水提物对小鼠强制游泳实验和悬尾实验的不动时间明显缩短。MTT实验显示，ZQ水提物及其主要成分（柚皮苷、橙皮苷、新橙皮苷和去皮苷）对皮质酮诱导的PC12细胞神经毒性具有神经保护作用。体内外实验结果表明，ZQ具有抗抑郁作用。
The immature fruit of Citrus aurantium L. (Zhi-Qiao, ZQ) has been used as a traditional medicine in China. Our previous study has shown that ZQ decoction may contribute to the antidepressant-like action of Chaihu-Shu-Gan-San. However, there are no reports on the chemical constituents of ZQ aqueous extract or its anti-depression effects. Firstly, this research reported the on-line identification of the chemical constituents in the aqueous extract of ZQ by coupling ultra-performance liquid chromatography/time-of-flight mass spectrometry (UPLC-Q-TOF/MS). A total of 31 chemical constituents were identified in ZQ aqueous extract, including one tannic acid, five flavones, 13 flavanones, one limonoid, three coumarins, three cyclic peptides, and five polymethoxylated flavonoids. The antidepressant effect of ZQ aqueous extract was evaluated in vivo and the results indicated that the mice immobility time during the forced swimming test and the tail suspension test were significantly reduced with ZQ treatment. MTT assays showed both ZQ aqueous extract and its major constituents (naringin, hesperidin, neohesperidin, and nobiletin) had neuroprotective effect on corticosterone-induced neurotoxicity in PC12 cells. The in vivo and in vitro results suggest that ZQ has an antidepressant effect.
登录
查看更多内容
代谢组学结合 UPLC-MS 化学特征发现中药柴胡舒肝散的抗抑郁成分
DOI：
10.1155/2013/487158
发表时间：
2013
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Jia H;Su Z;Long W;Liu Y;Chang X;Zhang H;Ding G;Feng Y;Cai D;Zou Z
通讯作者：
Zou Z
影响因子：
3.8
作者：
Donato, Franciele;de Gomes, Marcelo Gomes;Jesse, Cristiano Ricardo
通讯作者：
Jesse, Cristiano Ricardo
DOI：
10.1016/j.jpba.2011.02.013
发表时间：
2011-06-01
影响因子：
3.4
作者：
Su, Zhi-Heng;Li, Shu-Qi;Zou, Zhong-Mei
通讯作者：
Zou, Zhong-Mei
影响因子：
7.9
作者：
Herrera-Ruiz, M.;Zamilpa, A.;Huerta-Reyes, M.
通讯作者：
Huerta-Reyes, M.
影响因子：
3.4
作者：
STERU, L;CHERMAT, R;SIMON, P
通讯作者：
SIMON, P