Erythrocyte transketolase activity coefficient (ETKAC) assay protocol for the assessment of thiamine status.
Erythrocyte transketolase activity coefficient (ETKAC) assay protocol for the assessment of thiamine status.
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DOI：
10.1111/nyas.14547
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发表时间：
2021-08
影响因子：
5.2
通讯作者：
Koulman A
中科院分区：
文献类型：
--
作者：
Jones KS;Parkington DA;Cox LJ;Koulman A
维生素B1(硫胺素)是一种基本的营养物质，在许多代谢过程中起辅助作用，特别是在能量代谢过程中。典型的硫胺素缺乏的症状被认为是脚气病，尽管临床症状是非特异性的，亚临床缺乏的识别是困难的。因此，需要可靠的硫胺素状态生物标志物。硫胺素二磷酸是转酮醇酶的辅因子，包括红细胞转酮醇酶(ETK)。ETK活性测定作为硫胺素状态的间接功能标记物已经使用了50多年。ETK活性测定提供了硫胺素状态的敏感和特异的生物标志物；然而，对于缺乏的分界值缺乏共识，部分原因是缺乏分析协调。在这里，我们为ETK活性的测量和ETK活性系数的计算提供了一种循序渐进的方案，包括对所需设备和化学品的详细说明以及质量控制程序的指导。议定书的统一将为制定国际公认的硫胺素缺乏症分界线奠定基础。建议建立质量控制材料和质量保证方案，以提供可靠性。这将确保ETK活性分析仍然是评估硫胺状态的重要方法。需要可靠的硫胺素状态生物标志物来帮助诊断硫胺素缺乏症。硫胺素二磷酸(ThDP)是转酮醇酶的辅因子，包括红细胞转酮醇酶(ETK)。ETK活性测定提供了硫胺素状态的敏感和特异的生物标志物；然而，对于硫胺素缺乏症的界值缺乏共识，部分原因是缺乏分析协调。在这里，我们提供了一个逐步测量ETK活性和计算ETK活性系数(ETKAC)的方案。
Vitamin B1 (thiamine) is an essential nutrient that acts as a cofactor for a number of metabolic processes, particularly in energy metabolism. Symptoms of classic thiamine deficiency are recognized as beriberi, although clinical symptoms are nonspecific and recognition of subclinical deficiency is difficult. Therefore, reliable biomarkers of thiamine status are required. Thiamine diphosphate is a cofactor for transketolase, including erythrocyte transketolase (ETK). The ETK activity assay as an indirect, functional marker of thiamine status has been used for over 50 years. The ETK activity assay provides a sensitive and specific biomarker of thiamine status; however, there is a lack of consensus over the cutoffs for deficiency, partly due to a lack of assay harmonization. Here, we provide a step‐by‐step protocol for the measurement of ETK activity and the calculation of the ETK activity coefficient, including detailed explanations of equipment and chemicals required and guidance for quality control procedures. Harmonization of the protocol will provide the basis for the development of internationally recognized cutoffs for thiamine insufficiency. The establishment of quality control materials and a quality assurance scheme are recommended to provide reliability. This will ensure that the ETK activity assay remains an important method for the assessment of thiamine status. Reliable biomarkers of thiamine status are required to help diagnose thiamine deficiency. Thiamine diphosphate (ThDP) is a cofactor for transketolase, including erythrocyte transketolase (ETK). The ETK activity assay provides a sensitive and specific biomarker of thiamine status; however, there is a lack of consensus over the cutoffs for thiamine deficiency, partly due to a lack of assay harmonization. Here, we provide a step‐by‐step protocol for the measurement of ETK activity and the calculation of the ETK activity coefficient (ETKAC).
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影响因子：
2.2
作者：
MOUNT, JN;HEDUAN, E;MARSH, A
通讯作者：
MARSH, A
影响因子：
5
作者：
PRICE, J;CLAGUE, AE;NIXON, PF
通讯作者：
NIXON, PF
DOI：
10.1016/0305-0491(87)90397-x
发表时间：
1987-01-01
影响因子：
2.2
作者：
ALI, M;GUBLER, CJ;ABUFARSAK, F
通讯作者：
ABUFARSAK, F
影响因子：
158.5
作者：
DREYFUS, PM
通讯作者：
DREYFUS, PM
影响因子：
4.2
作者：
BRIN, M
通讯作者：
BRIN, M