Association of single nucleotide polymorphic sites in candidate genes with aggressiveness and deoxynivalenol production in Fusarium graminearum causing wheat head blight.
Association of single nucleotide polymorphic sites in candidate genes with aggressiveness and deoxynivalenol production in Fusarium graminearum causing wheat head blight.
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DOI：
10.1186/1471-2156-13-14
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发表时间：
2012-03-12
期刊：
影响因子：
2.9
通讯作者：
Miedaner T
中科院分区：
文献类型：
--
作者：
Talas F;Würschum T;Reif JC;Parzies HK;Miedaner T
禾谷镰刀菌 sensu stricto (s.s.) 是谷物中普遍存在的病原体。赤霉病 (FHB) 的经济影响表现为农作物损失和霉菌毒素污染。我们的目标是将候选基因内的 SNP 多样性与表型性状关联起来。总共 77 个 F. graminearum s.s.每两年在两个地点进行接种田间实验，测试分离株的真菌感染严重程度（=侵袭性）和脱氧雪腐镰刀菌烯醇（DON）产量。对于已知控制真菌生长（MetAP1、Erf2）或 DON 产生（TRI1、TRI5、TRI6、TRI10 和 TRI14）的 7 个基因，确定了单核苷酸多态性位点 (SNP)，并评估了连锁不平衡 (LD) 的程度。使用线性混合模型测试了 SNP 与两种表型性状的关联。在大多数情况下，LD 的衰减速度很快。 MetAP1 中的两个相邻 SNP 和 Erf2 中的一个 SNP 与攻击性显着相关 (P < 0.05)，这解释了基因型变异 (pG) 的比例分别为 25.6%、0.5% 和 13.1%。 TRI1 中的一个 SNP 与 DON 产生显着相关 (pG = 4.4)。我们认为，以已发表的禾谷镰刀菌序列信息为模板，扩增候选基因的比较序列部分是检测数量性状位点的有效方法。我们的研究结果强调了候选基因关联作图方法在识别禾谷镰刀菌种群攻击性和 DON 产生的功能性 SNP 方面的潜力。
Fusarium graminearum sensu stricto (s.s.) is an ubiquitous pathogen of cereals. The economic impact of Fusarium head blight (FHB) is characterized by crop losses and mycotoxin contamination. Our objective was to associate SNP diversity within candidate genes with phenotypic traits. A total of 77 F. graminearum s.s. isolates was tested for severity of fungal infection (= aggressiveness) and deoxynivalenol (DON) production in an inoculated field experiment at two locations in each of two years. For seven genes known to control fungal growth (MetAP1, Erf2) or DON production (TRI1, TRI5, TRI6 TRI10 and TRI14) single nucleotides polymorphic sites (SNPs) were determined and evaluated for the extent of linkage disequilibrium (LD). Associations of SNPs with both phenotypic traits were tested using linear mixed models. Decay of LD was in most instances fast. Two neighboring SNPs in MetAP1 and one SNP in Erf2 were significantly (P < 0.05) associated with aggressiveness explaining proportions of genotypic variance (pG) of 25.6%, 0.5%, and 13.1%, respectively. One SNP in TRI1 was significantly associated with DON production (pG = 4.4). We argue that using the published sequence information of Fusarium graminearum as a template to amplify comparative sequence parts of candidate genes is an effective method to detect quantitative trait loci. Our findings underline the potential of candidate gene association mapping approaches to identify functional SNPs underlying aggressiveness and DON production for F. graminearum s.s populations.
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影响因子：
5.4
作者：
HILL W G;ROBERTSON A
通讯作者：
ROBERTSON A
影响因子：
3.5
作者：
Bluhm, B. H.;Zhao, X.;Dunkle, L. D.
通讯作者：
Dunkle, L. D.
影响因子：
6.1
作者：
Dyer, RB;Plattner, RD;Brown, DW
通讯作者：
Brown, DW
影响因子：
5.4
作者：
BERNARDO, R
通讯作者：
BERNARDO, R
影响因子：
3.2
作者：
Cumagun, CJR;Bowden, RL;Miedaner, T
通讯作者：
Miedaner, T