Isolation and Characterization of Human Lung Lymphatic Endothelial Cells.
Isolation and Characterization of Human Lung Lymphatic Endothelial Cells.
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DOI：
10.1155/2015/747864
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发表时间：
2015
影响因子：
--
通讯作者：
Quaini F
中科院分区：
文献类型：
--
作者：
Lorusso B;Falco A;Madeddu D;Frati C;Cavalli S;Graiani G;Gervasi A;Rinaldi L;Lagrasta C;Maselli D;Gnetti L;Silini EM;Quaini E;Ampollini L;Carbognani P;Quaini F
来自呼吸系统的淋巴管内皮细胞的特征对于研究淋巴系统在正常和病变肺中的作用可能是至关重要的。我们描述了一种简单而廉价的方法来获取、分离和扩增人肺淋巴管内皮细胞(HL-LECs)。本文对55例肺叶切除术患者的健康肺标本进行了研究。采用抗CD31和泊多拉宁的单抗进行顺磁分选的两步纯化工具，筛选出纯种HL-LECs。通过形态、免疫细胞化学、流式细胞术和功能分析鉴定HL-LECs的纯度。有趣的是，这些细胞在体外保留了几个与细胞生存和增殖有关的受体酪氨酸激酶(RTK)。HL-LECs是一种临床相关的细胞基质，用于研究淋巴管生物学、淋巴管生成、与微生物的相互作用、伤口愈合和抗癌治疗。
Characterization of lymphatic endothelial cells from the respiratory system may be crucial to investigate the role of the lymphatic system in the normal and diseased lung. We describe a simple and inexpensive method to harvest, isolate, and expand lymphatic endothelial cells from the human lung (HL-LECs). Fifty-five samples of healthy lung selected from patients undergoing lobectomy were studied. A two-step purification tool, based on paramagnetic sorting with monoclonal antibodies to CD31 and Podoplanin, was employed to select a pure population of HL-LECs. The purity of HL-LECs was assessed by morphologic criteria, immunocytochemistry, flow cytometry, and functional assays. Interestingly, these cells retain in vitro several receptor tyrosine kinases (RTKs) implicated in cell survival and proliferation. HL-LECs represent a clinically relevant cellular substrate to study lymphatic biology, lymphoangiogenesis, interaction with microbial agents, wound healing, and anticancer therapy.
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影响因子：
11.5
作者：
Chitnis, Meenali M.;Yuen, John S. P.;Macaulay, Valentine M.
通讯作者：
Macaulay, Valentine M.
影响因子：
20.1
作者：
Caporali, Andrea;Pani, Elisabetta;Emanueli, Costanza
通讯作者：
Emanueli, Costanza
影响因子：
7.8
作者：
Banerji, S;Ni, J;Wang, S X;Clasper, S;Su, J;Tammi, R;Jones, M;Jackson, D G
通讯作者：
Jackson, D G
影响因子：
4
作者：
Catravas, John D.;Snead, Connie;Dimitropoulou, Christiana;Chang, Albert S. Y.;Lucas, Rudolf;Verin, Alexander D.;Black, Stephen M.
通讯作者：
Black, Stephen M.
影响因子：
5.4
作者：
Aird, William C.
通讯作者：
Aird, William C.