Species-Level Spatio-Temporal Dynamics of Cyanobacteria in a Hard-Water Temperate Lake in the Southern Baltics.
Species-Level Spatio-Temporal Dynamics of Cyanobacteria in a Hard-Water Temperate Lake in the Southern Baltics.
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DOI：
10.3389/fmicb.2021.761259
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发表时间：
2021
影响因子：
5.2
通讯作者：
Liebner S
中科院分区：
文献类型：
--
作者：
Nwosu EC;Roeser P;Yang S;Pinkerneil S;Ganzert L;Dittmann E;Brauer A;Wagner D;Liebner S
蓝藻是温带淡水生态系统中重要的初级生产者。然而，基于高通量DNA测序的深海湖泊蓝藻季节和空间分布的研究还很少见。在这项研究中，我们结合了2019年每月的水质采样和监测，扩增子序列变体分析（ASV;不同物种的代理）和针对整体蓝藻丰度的定量PCR，以描述德国东北部的铁弗湖（Tiefer See）深水贫中营养蓝藻的季节和空间动态。我们观察到显着的季节变化的蓝藻群落组成（p < 0.05）在表水层和变水层，但不是在低层。在冬季，当水柱混合时，微蓝细菌（聚球藻和蓝藻）占优势。随着春末分层的开始，我们观察到潜在的生态位专业化和蓝细菌类群之间的共存主要是由光和营养动态驱动。具体而言，ASV分配到picocyanobacteria和属Planktothrix的主要贡献者形成的深叶绿素最大值沿着光梯度。而聚球藻和不同的蓝藻ASVs丰富的上层直到底部的真光区从春季到秋季，浮游发藻主要发生在metalimnetic层下面的真光区，也整体蓝藻丰度是最高的夏季。我们的数据显示，两个潜在的psychrotolerant（冷适应）Cyanobium物种，似乎科普以及在较低的hypolimnetic水温和光照条件下，以及增加沉积物释放的磷酸盐在更深的沃茨在夏季。潜在的冷适应蓝藻物种也占主导地位，整个水柱在秋季和冬季。此外，Snowella和微囊藻相关的ASVs丰富的水柱在秋季营业额的发病。总之，这些研究结果表明，以前未确定的和相当大的时空变化，特别是在深硬水温带湖泊蓝藻属蓝藻群落的物种水平。
Cyanobacteria are important primary producers in temperate freshwater ecosystems. However, studies on the seasonal and spatial distribution of cyanobacteria in deep lakes based on high-throughput DNA sequencing are still rare. In this study, we combined monthly water sampling and monitoring in 2019, amplicon sequence variants analysis (ASVs; a proxy for different species) and quantitative PCR targeting overall cyanobacteria abundance to describe the seasonal and spatial dynamics of cyanobacteria in the deep hard-water oligo-mesotrophic Lake Tiefer See, NE Germany. We observed significant seasonal variation in the cyanobacterial community composition (p < 0.05) in the epi- and metalimnion layers, but not in the hypolimnion. In winter—when the water column is mixed—picocyanobacteria (Synechococcus and Cyanobium) were dominant. With the onset of stratification in late spring, we observed potential niche specialization and coexistence among the cyanobacteria taxa driven mainly by light and nutrient dynamics. Specifically, ASVs assigned to picocyanobacteria and the genus Planktothrix were the main contributors to the formation of deep chlorophyll maxima along a light gradient. While Synechococcus and different Cyanobium ASVs were abundant in the epilimnion up to the base of the euphotic zone from spring to fall, Planktothrix mainly occurred in the metalimnetic layer below the euphotic zone where also overall cyanobacteria abundance was highest in summer. Our data revealed two potentially psychrotolerant (cold-adapted) Cyanobium species that appear to cope well under conditions of lower hypolimnetic water temperature and light as well as increasing sediment-released phosphate in the deeper waters in summer. The potential cold-adapted Cyanobium species were also dominant throughout the water column in fall and winter. Furthermore, Snowella and Microcystis-related ASVs were abundant in the water column during the onset of fall turnover. Altogether, these findings suggest previously unascertained and considerable spatiotemporal changes in the community of cyanobacteria on the species level especially within the genus Cyanobium in deep hard-water temperate lakes.
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影响因子：
2.1
作者：
Draeger, Nadine;Plessen, Birgit;Brauer, Achim
通讯作者：
Brauer, Achim
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
3.7
作者：
Bushnell B;Rood J;Singer E
通讯作者：
Singer E
影响因子：
1.4
作者：
Choi, Dong Han;Noh, Jae Hoon;Shim, JaeSeol
通讯作者：
Shim, JaeSeol
影响因子：
1.6
作者：
Cai, Ji;Hodoki, Yoshikuni;Nakano, Shin-ichi
通讯作者：
Nakano, Shin-ichi