Aluminum is a weak agonist for the calcium-sensing receptor.
Aluminum is a weak agonist for the calcium-sensing receptor.
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铝是钙敏感受体的弱激动剂。
DOI：
10.1046/j.1523-1755.1999.00432.x
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Quarles,LD
中科院分区：
文献类型：
--
作者：
Spurney,RF;Pi,M;Flannery,P;Quarles,LD
研究背景铝（Al 3+）通过激活细胞外阳离子敏感受体介导多种生物学效应。最近发现的一种钙敏感受体（CaSR），已在Al 3+的靶组织中被鉴定，可能与Al 3+的一些生物学效应有关。我们用编码大鼠CaSR的cDNA转染人胚肾293（HEK 293）细胞，通过Western印迹分析评估CaSR表达，并通过测量细胞内钙（[Ca 2 +]i）来评估CaSR功能。水平和肌醇一磷酸（IP 1）的产生刺激后，铝和一个面板的CaSR agonists.ResultsThe CaSR蛋白的免疫印迹分析检测到转染的细胞中的CaSR cDNA，但不是在非转染HEK 293细胞。此外，[Ca 2 +] i水平和IP 1的产生以剂量依赖性的方式增加的CaSR激动剂钙（Ca 2+），镁（Mg 2+），钆（Gd 3+），和新霉素仅在细胞转染CaSR。为了确定Al 3+是否激活CaSR，我们用10 μ m至1 mm浓度的Al 3+刺激转染了大鼠CaSR的细胞。Al ~（3+）浓度在10 μ m ~ 100 μ m范围内对[Ca ~（2+）] i水平和IP_1的产生无影响。相比之下，1毫米铝3+诱导小，但在这两个参数显着增加。Gd 3+可拮抗Ca 2+对CaSR的激活作用，而Al 3+预处理则不能阻断Ca 2+对CaSR的激活作用，提示Al 3+的作用机制是不同的。由于Al 3+在微摩尔浓度下影响多种靶组织，CaSR似乎不太可能介导Al 3+的这些细胞作用。
Aluminum is a weak agonist for the calcium-sensing receptor.BackgroundAluminum (Al3+) has diverse biological effects mediated through activation of a putative extracellular cation-sensing receptor. A recently identified calcium-sensing receptor (CaSR), which has been identified in target tissues for Al3+, may transduce some of the biological effects of Al3+.MethodsTo test this possibility, we transfected human embryonic kidney 293 (HEK 293) cells with a cDNA encoding the rat CaSR and evaluated CaSR expression by Western blot analysis and function by measurement of intracellular calcium ([Ca2+]i) levels and inositol monophosphate (IP1) generation following stimulation with Al3+and a panel of CaSR agonists.ResultsThe CaSR protein was detected by immunoblot analysis in cells transfected with the CaSR cDNA but not in nontransfected HEK 293 cells. In addition, [Ca2+]ilevels and IP1 generation were enhanced in a dose-dependent fashion by additions of the CaSR agonists calcium (Ca2+), magnesium (Mg2+), gadolinium (Gd3+), and neomycin only in cells transfected with CaSR. To determine if Al3+activated CaSR, we stimulated cells transfected with rat CaSR with 10 μmto 1 mmconcentrations of Al3+. Concentrations of Al3+in the range of 10 μmto 100 μmhad no effect on [Ca2+]ilevels or IP1 generation. In contrast, 1 mmAl3+induced small but significant increases in both parameters. Whereas Gd3+antagonized calcium-mediated activation of CaSR, pretreatment with Al3+failed to block subsequent activation of rat CaSR by Ca2+, suggesting a distinct mechanism of Al3+action.ConclusionAl3+is not a potent agonist for CaSR. Because Al3+affects a variety of target tissues at micromolar concentrations, it seems unlikely that CaSR mediates these cellular actions of Al3+.
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铝离子刺激组织培养中小鼠细胞的有丝分裂
影响因子：
4
作者：
T. Jones;Donna L. Antonetti;T. Reid
通讯作者：
T. Reid
DOI：
--
发表时间：
1985-03
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
G. Grynkiewicz;M. Poenie;Roger Y. TsienB
通讯作者：
G. Grynkiewicz;M. Poenie;Roger Y. TsienB
影响因子：
4.8
作者：
QUARLES, LD;WENSTRUP, RJ;DREZNER, MK
通讯作者：
DREZNER, MK
DOI：
10.1073/pnas.79.16.4888
发表时间：
1982-08
影响因子：
11.1
作者：
P. Sternweis;A. Gilman
通讯作者：
P. Sternweis;A. Gilman
长期接触铝会损害神经元谷氨酸-一氧化氮-环 GMP 通路
影响因子：
4.7
作者：
C. Cucarella;C. Montoliu;C. Hermenegildo;R. Sáez;L. Manzo;M. Miñana;V. Felipo
通讯作者：
V. Felipo