Development of a micro-tissue-mediated injectable bone tissue engineering strategy for large segmental bone defect treatment.
Development of a micro-tissue-mediated injectable bone tissue engineering strategy for large segmental bone defect treatment.
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开发用于大节段骨缺损治疗的微组织介导的可注射骨组织工程策略
DOI：
10.1186/s13287-018-1064-1
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发表时间：
2018-11-28
影响因子：
7.5
通讯作者：
Zhang ZY
中科院分区：
文献类型：
--
作者：
Wu D;Wang Z;Wang J;Geng Y;Zhang Z;Li Y;Li Q;Zheng Z;Cao Y;Zhang ZY
背景骨组织工程在临床治疗中应用并不广泛。这背后隐藏着两个主要原因：(1)种子细胞难以获得；(2)组织工程骨构建过程过于复杂，效率仍然相对较低。可以预见，在不久的将来，种子细胞来源的问题在组织工程骨修复中可以得到彻底解决。针对组织工程骨构建过程复杂、效率低的问题，通常考虑两种策略：(1)基于可注射骨组织的构建策略和(2)基于成骨细胞片的构建策略。然而，可注射骨组织工程（iBTE）策略和成骨细胞片策略的应用有限，难以直接用于大节段骨，特别是承重骨的缺损修复。方法在本研究中，我们构建了结构简单，但具有一定结构和成分的成骨微组织。在此基础上，我们建立了一种新的iBTE修复策略——成骨微组织原位修复策略，主要针对解决大节段性骨缺损问题。步骤如下：(1)根据缺陷部位的大小，采用3D打印快速成型技术构建可生物降解的三维支架。(2)将三维支架植入缺损部位。这种支架被认为是既能提供机械支撑又能提供骨组织生长空间的“钢框架”。(3)将可视为“混凝土”的成骨微组织（即“细胞-细胞外基质”复合物）注入三维支架中，促进骨组织原位再生。同时注射消化后的细胞作为对照组。3个月后，比较成骨微组织和消化细胞原位修复骨缺损的效果。结果与消化细胞相比，成骨微组织具有更高的细胞利用效率和更好的修复效果。结论成骨微组织修复策略是解决大节段骨缺损的一种较有前途的临床策略。
BackgroundBone tissue engineering is not widely used in clinical treatment. Two main reasons hide behind this: (1) the seed cells are difficult to obtain and (2) the process of tissue engineering bone construction is too complex and its efficiency is still relatively low. It is foreseeable that in the near future, the problem of seed cell sources could be solved completely in tissue engineering bone repair. As for the complex process and low efficiency of tissue engineering bone construction, usually two strategies would be considered: (1) the construction strategy based on injectable bone tissue and (2) the construction strategy based on osteogenic cell sheets. However, the application of injectable bone tissue engineering (iBTE) strategy and osteogenic cell sheet strategy is limited and they could hardly be used directly in repairing defects of large segmental bone, especially load-bearing bone.MethodsIn this study, we built an osteogenic micro-tissue with simple construction but with a certain structure and composition. Based on this, we established a new iBTE repair strategy—osteogenic micro-tissue in situ repair strategy, mainly targeting at solving the problem of large segmental bone defect. The steps are as follows: (1) Build the biodegradable three-dimensional scaffold based on the size of the defect site with 3D printing rapid prototyping technology. (2) Implant the three-dimensional scaffold into the defect site. This scaffold is considered as the “steel framework” that could provide both mechanical support and space for bone tissue growth. (3) Inject the osteogenic micro-tissue (i.e., the “cell-extracellular matrix” complex), which could be considered as “concrete,” into the three-dimensional scaffold, to promote the bone tissue regeneration in situ. Meanwhile, the digested cells were injected as the compared group in this experiment. After 3 months, the effect of in situ bone defect repair of osteogenic micro-tissue and digested cells was compared.ResultsIt is confirmed that osteogenic micro-tissue could achieve a higher efficiency on cell usage and has a better repair effect than the digested cells.ConclusionsOsteogenic micro-tissue repairing strategy would be a more promising clinical strategy to solve the problem of large segmental bone defect.
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影响因子：
14
作者：
Nichol, Jason W.;Koshy, Sandeep T.;Bae, Hojae;Hwang, Chang M.;Yamanlar, Seda;Khademhosseini, Ali
通讯作者：
Khademhosseini, Ali
DOI：
10.1083/jcb.113.3.681
发表时间：
1991-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Yamaguchi A;Katagiri T;Ikeda T;Wozney JM;Rosen V;Wang EA;Kahn AJ;Suda T;Yoshiki S
通讯作者：
Yoshiki S
影响因子：
5.2
作者：
Yamada, Yoichi;Nakamura, Sayaka;Ito, Kenji;Umemura, Eri;Hara, Kenji;Nagasaka, Tetsuro;Abe, Akihiro;Baba, Shunsuke;Furuichi, Yasushi;Izumi, Yuichi;Klein, Ophir D.;Wakabayashi, Toshihiko
通讯作者：
Wakabayashi, Toshihiko
在犬模型中用脂肪干细胞和珊瑚支架修复颅骨缺损
影响因子：
14
作者：
Cui, Lei;Liu, Bo;Cao, Yilin
通讯作者：
Cao, Yilin
影响因子：
1.1
作者：
Wang Z;Cao F;Liu D;Fang Y;Li F
通讯作者：
Li F