Novel and conserved protein macoilin is required for diverse neuronal functions in Caenorhabditis elegans.
Novel and conserved protein macoilin is required for diverse neuronal functions in Caenorhabditis elegans.
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DOI：
10.1371/journal.pgen.1001384
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发表时间：
2011-05
期刊：
影响因子：
4.5
通讯作者：
Mori I
中科院分区：
文献类型：
--
作者：
Miyara A;Ohta A;Okochi Y;Tsukada Y;Kuhara A;Mori I
神经信号在动物的神经系统中处理，以响应可变的环境刺激。这项研究表明，一种新的和高度保守的蛋白质，macoilin（MACO-1），在秀丽隐杆线虫的各种神经功能中起着至关重要的作用。maco-1突变体表现出异常的行为，包括有缺陷的运动、趋热性和趋化性。人macoilin在C. elegans神经系统微弱地挽救了maco-1突变体的异常趋热表型，表明macoilin在物种间是功能保守的。异常的趋热性可能是由maco-1突变体的运动受损引起的。然而，钙成像的AFD热感觉神经元和AIY突触后中间神经元的maco-1突变体表明，macoilin是必要的AFD和AIY神经元热刺激的适当反应。MACO-1定位研究表明，C.线虫和人macoilins主要定位于粗面内质网。我们的研究结果表明，macoilin是所需的各种神经活动，如神经元活动的调节。任何动物，包括人类，都必须能够正确地感知和响应各种环境刺激，以生存和繁殖。环境刺激会被评估，并根据过去的经验进行转换以产生适当的适应行为。即使是小型的、自由生活的土壤线虫秀丽隐杆线虫（Caushabditis elegans）也可以使用仅由302个神经元组成的神经系统来感知各种环境刺激。C.秀丽线虫表现出趋热性，这使它们能够记住周围的温度，并利用这一信息作为寻找和保持在食物来源附近的线索。我们在这里表明，一种新的和保守的蛋白质，MACO-1，是重要的适当执行的趋热性由C。优雅maco-1基因最初是在一种新的趋热性缺陷突变体中发现的。MACO-1突变体的异常趋热性弱，但显然获救表达人类MACO-1，这表明MACO-1是物种之间的功能保守。MACO-1蛋白是适当调节神经元活性所必需的，并且在MACO-1突变体中，趋热性所需的神经元活性受损。这一分析为macoilin家族的功能提供了重要依据。
Neural signals are processed in nervous systems of animals responding to variable environmental stimuli. This study shows that a novel and highly conserved protein, macoilin (MACO-1), plays an essential role in diverse neural functions in Caenorhabditis elegans. maco-1 mutants showed abnormal behaviors, including defective locomotion, thermotaxis, and chemotaxis. Expression of human macoilin in the C. elegans nervous system weakly rescued the abnormal thermotactic phenotype of the maco-1 mutants, suggesting that macoilin is functionally conserved across species. Abnormal thermotaxis may have been caused by impaired locomotion of maco-1 mutants. However, calcium imaging of AFD thermosensory neurons and AIY postsynaptic interneurons of maco-1 mutants suggest that macoilin is required for appropriate responses of AFD and AIY neurons to thermal stimuli. Studies on localization of MACO-1 showed that C. elegans and human macoilins are localized mainly to the rough endoplasmic reticulum. Our results suggest that macoilin is required for various neural events, such as the regulation of neuronal activity. Any animals, including humans, have to be capable of properly sensing and responding to various environmental stimuli for survival and reproduction. Environmental stimuli are evaluated and, based on past experiences, converted to produce appropriate adaptive behaviors. Even the small, free-living soil nematode Caenorhabditis elegans can sense diverse environment stimuli using a nervous system that consists of only 302 neurons. C. elegans exhibit thermotaxis that allows them to remember ambient temperatures and use this information as a cue to seek and remain near food sources. We show here that a novel and conserved protein, MACO-1, is important for proper execution of thermotaxis by C. elegans. The maco-1 gene was originally identified in a novel thermotaxis-defective mutant. Abnormal thermotaxis of maco-1 mutants was weakly but apparently rescued by expressing human MACO-1, suggesting that MACO-1 is functionally conserved among species. MACO-1 protein is required for appropriate regulation of neuronal activity, and the activity of neurons required for thermotaxis is impaired in maco-1 mutants. This analysis provides an important basis for the function of the macoilin family.
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影响因子：
64.5
作者：
BARGMANN, CI;HARTWIEG, E;HORVITZ, HR
通讯作者：
HORVITZ, HR
影响因子：
2.7
作者：
Fukushige, T;Schroeder, DF;McGhee, JD
通讯作者：
McGhee, JD
影响因子：
16.2
作者：
Gomez, M;De Castro, E;Nef, P
通讯作者：
Nef, P
影响因子：
3
作者：
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通讯作者：
Schafer, WR
影响因子：
56.9
作者：
Kuhara, Atsushi;Okumura, Masatoshi;Mori, Ikue
通讯作者：
Mori, Ikue