Mass spectrometry of fatty aldehydes.
Mass spectrometry of fatty aldehydes.
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DOI：
10.1016/j.bbalip.2011.08.018
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发表时间：
2011-11
期刊：
影响因子：
--
通讯作者：
Berdyshev EV
中科院分区：
文献类型：
--
作者：
Berdyshev EV
脂肪醛是细胞脂质组的重要组成部分。由于多不饱和脂肪酸氧化而形成的短链α、β-不饱和醛和羟基化醛的分析引起了人们的极大兴趣。多种气相色谱-质谱（GC/MS）和随后的液相色谱-质谱（LC/MS）方法已被开发用于鉴定和量化短链和长链脂肪醛。由于游离醛能够与蛋白质、脂质和DNA胍的氨基非酶性形成希夫碱，游离醛被视为脂肪酸氧化的标记或度量，而不是细胞内信号通路的一部分，这极大地限制了这组分子得到的总体关注。本文综述了目前脂肪醛分析的GC/MS和LC/MS方法，并讨论了游离脂肪醛定量方法中存在的技术挑战。
Fatty aldehydes are important components of the cellular lipidome. Significant interest has been developed towards the analysis of the short chain α,β-unsaturated and hydroxylated aldehydes formed as a result of oxidation of polyunsaturated fatty acids. Multiple gas chromatography-mass spectrometry (GC/MS) and subsequently liquid chromatography-mass spectrometry (LC/MS) approaches have been developed to identify and quantify short-chain as well as long-chain fatty aldehydes. Due to the ability to non-enzymaticaly form Schiff bases with amino groups of proteins, lipids, and with DNA guanidine, free aldehydes are viewed as a marker or metric of fatty acid oxidation and not the part of intracellular signaling pathways which has significantly limited the overall attention this group of molecules have received. This review provides an overview of current GC/MS and LC/MS approaches of fatty aldehyde analysis as well as discusses technical challenges standing in the way of free fatty aldehyde quantitation.
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影响因子：
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