Region-specific irradiation system with heavy-ion microbeam for active individuals of Caenorhabditis elegans.
Region-specific irradiation system with heavy-ion microbeam for active individuals of Caenorhabditis elegans.
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DOI：
10.1093/jrr/rrx043
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发表时间：
2017-11-01
影响因子：
2
通讯作者：
Funayama T
中科院分区：
文献类型：
--
作者：
Suzuki M;Hattori Y;Sakashita T;Yokota Y;Kobayashi Y;Funayama T
辐射可能会影响基本功能和行为，例如运动、进食、学习和记忆。尽管全身照射已被证明可以降低线虫秀丽隐杆线虫的运动性，但造成这种效应的详细机制仍不清楚。对负责感觉统合和信息处理的神经环进行靶向照射，将使我们能够确定全身照射后运动性的降低是否反映了对中枢神经系统或肌肉细胞本身的影响。因此，我们使用准直微束系统解决了这个问题。然而，辐射靶向需要固定动物，并且之前的研究已经麻醉动物以防止其运动，因此不可能在照射后立即评估它们的运动情况。我们开发了一种方法，将动物封闭在聚二甲基硅氧烷芯片的直微流体通道中，以抑制照射期间的自由运动，从而允许在照射后立即观察运动。头部区域（包括中枢神经系统）、肠道和子宫周围的中部区域以及尾部区域被独立瞄准。每个区域均受到 12 000 个碳离子（12C；18.3 MeV/u；线性能量转移 = 106.4 keV/μm）的照射，相当于 φ20 μm 区域的 500 Gy。全身照射会显着降低运动能力，但对任何单个区域（包括中枢神经系统）的照射不会显着降低运动能力。这表明辐射通过全身机制抑制运动，可能涉及运动神经元和/或体壁肌肉细胞，而不是通过中枢神经系统和刺激反应影响运动控制。
Radiation may affect essential functions and behaviors such as locomotion, feeding, learning and memory. Although whole-body irradiation has been shown to reduce motility in the nematode Caenorhabditis elegans, the detailed mechanism responsible for this effect remains unknown. Targeted irradiation of the nerve ring responsible for sensory integration and information processing would allow us to determine whether the reduction of motility following whole-body irradiation reflects effects on the central nervous system or on the muscle cells themselves. We therefore addressed this issue using a collimating microbeam system. However, radiation targeting requires the animal to be immobilized, and previous studies have anesthetized animals to prevent their movement, thus making it impossible to assess their locomotion immediately after irradiation. We developed a method in which the animal was enclosed in a straight, microfluidic channel in a polydimethylsiloxane chip to inhibit free motion during irradiation, thus allowing locomotion to be observed immediately after irradiation. The head region (including the central nervous system), mid region around the intestine and uterus, and tail region were targeted independently. Each region was irradiated with 12 000 carbon ions (12C; 18.3 MeV/u; linear energy transfer = 106.4 keV/μm), corresponding to 500 Gy at a φ20 μm region. Motility was significantly decreased by whole-body irradiation, but not by irradiation of any of the individual regions, including the central nervous system. This suggests that radiation inhibits locomotion by a whole-body mechanism, potentially involving motoneurons and/or body-wall muscle cells, rather than affecting motor control via the central nervous system and the stimulation response.
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影响因子：
2
作者：
Miyazawa, Yutaka;Sakashita, Tetsuya;Takahashi, Hideyuki
通讯作者：
Takahashi, Hideyuki
影响因子：
2
作者：
Funayama, Tornoo;Wada, Seiichi;Kobayashi, Yasuhiko
通讯作者：
Kobayashi, Yasuhiko
线虫中辐射诱导的从体细胞到生殖细胞的旁观者信号传导
影响因子：
3.4
作者：
Guo, Xiaoying;Sun, Jie;Wu, Lijun
通讯作者：
Wu, Lijun
影响因子：
2
作者：
Fukamoto, Kana;Shirai, Koji;Kiguchi, Kenji
通讯作者：
Kiguchi, Kenji
DOI：
10.2187/bss.17.298
发表时间：
2003-12-01
期刊：
Uchu Seibutsu Kagaku
影响因子：
--
作者：
Yokota, Yuichiro;Funayama, Tomoo;Inoue, Masayoshi
通讯作者：
Inoue, Masayoshi