DaXi-high-resolution, large imaging volume and multi-view single-objective light-sheet microscopy.
DaXi-high-resolution, large imaging volume and multi-view single-objective light-sheet microscopy.
复制标题
DOI：
10.1038/s41592-022-01417-2
复制
发表时间：
2022-04
期刊：
影响因子：
48
通讯作者：
Royer, Loic A.
中科院分区：
文献类型：
--
作者：
Yang, Bin;Lange, Merlin;Millett-Sikking, Alfred;Zhao, Xiang;Bragantini, Jordao;VijayKumar, Shruthi;Kamb, Mason;Gomez-Sjoberg, Rafael;Solak, Ahmet Can;Wang, Wanpeng;Kobayashi, Hirofumi;McCarroll, Matthew N.;Whitehead, Lachlan W.;Fiolka, Reto P.;Kornberg, Thomas B.;York, Andrew G.;Royer, Loic A.
单物镜光片显微镜的承诺是联合收割机结合的速度，覆盖面，分辨率和温和的光片显微镜的标准单物镜显微镜的方便。我们提出了DaXi，一种基于斜平面照明的单物镜光片显微镜设计，它实现了：（1）通过定制的远程聚焦物镜实现更宽的视场和高分辨率成像;（2）在不影响图像质量或需要平铺采集的情况下，在更大体积上实现快速体积成像;（3）通过多视图成像对大样品的更全面的图像覆盖，以及（4）通过远程盖玻片放置的更高通量的多孔成像。该仪器在3,000 × 800 × 300 μm的成像体积上实现了横向450 nm和轴向2 μm的分辨率。我们通过对各种系统进行成像来展示我们仪器的速度、视野、分辨率和多功能性，包括果蝇卵室发育、斑马鱼全脑活动和斑马鱼胚胎发育-一次最多可达9个胚胎。DaXi单物镜光片显微镜可实现快速、高质量的大体积成像。DaXi的设计允许在不牺牲成像速度或质量的情况下增加扫描范围，多视图成像和多功能样品安装。
The promise of single-objective light-sheet microscopy is to combine the convenience of standard single-objective microscopes with the speed, coverage, resolution and gentleness of light-sheet microscopes. We present DaXi, a single-objective light-sheet microscope design based on oblique plane illumination that achieves: (1) a wider field of view and high-resolution imaging via a custom remote focusing objective; (2) fast volumetric imaging over larger volumes without compromising image quality or necessitating tiled acquisition; (3) fuller image coverage for large samples via multi-view imaging and (4) higher throughput multi-well imaging via remote coverslip placement. Our instrument achieves a resolution of 450 nm laterally and 2 μm axially over an imaging volume of 3,000 × 800 × 300 μm. We demonstrate the speed, field of view, resolution and versatility of our instrument by imaging various systems, including Drosophila egg chamber development, zebrafish whole-brain activity and zebrafish embryonic development – up to nine embryos at a time. The DaXi single-objective light-sheet microscope achieves fast, high-quality imaging of large volumes. DaXi’s design allows increased scanning range without sacrificing imaging speed or quality, multiview imaging and versatile sample mounting.
登录
查看更多内容
DOI：
10.1126/science.aaq1392
发表时间：
2018-04-20
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Liu TL;Upadhyayula S;Milkie DE;Singh V;Wang K;Swinburne IA;Mosaliganti KR;Collins ZM;Hiscock TW;Shea J;Kohrman AQ;Medwig TN;Dambournet D;Forster R;Cunniff B;Ruan Y;Yashiro H;Scholpp S;Meyerowitz EM;Hockemeyer D;Drubin DG;Martin BL;Matus DQ;Koyama M;Megason SG;Kirchhausen T;Betzig E
通讯作者：
Betzig E
影响因子：
3.4
作者：
Dean, Kevin M.;Roudot, Philippe;Welf, Erik S.;Danuser, Gaudenz;Fiolka, Reto
通讯作者：
Fiolka, Reto
影响因子：
10.4
作者：
Hoffmann, Maximilian;Judkewitz, Benjamin
通讯作者：
Judkewitz, Benjamin
高级成像 内吞和细胞骨架动力学的扩展分辨率结构照明成像
DOI：
10.1126/science.aab3500
发表时间：
2015-08-28
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Li D;Shao L;Chen BC;Zhang X;Zhang M;Moses B;Milkie DE;Beach JR;Hammer JA 3rd;Pasham M;Kirchhausen T;Baird MA;Davidson MW;Xu P;Betzig E
通讯作者：
Betzig E
影响因子：
28.1
作者：
Glaser, Adam K.;Reder, Nicholas P.;Liu, Jonathan T. C.
通讯作者：
Liu, Jonathan T. C.