Widely Used Mutants of eiger, Encoding the Drosophila Tumor Necrosis Factor, Carry Additional Mutations in the NimrodC1 Phagocytosis Receptor.
Widely Used Mutants of eiger, Encoding the Drosophila Tumor Necrosis Factor, Carry Additional Mutations in the NimrodC1 Phagocytosis Receptor.
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DOI：
10.1534/g3.120.401800
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发表时间：
2020-12-03
期刊：
影响因子：
--
通讯作者：
Johnston LA
中科院分区：
文献类型：
--
作者：
Kodra A;de la Cova C;Gerhold AR;Johnston LA
上皮细胞凋亡的过程涉及半胱天冬酶的激活、细胞分层和细胞成分的降解。尸体和细胞碎片然后被吞噬性血细胞从组织中迅速清除。在果蝇TNF，Eiger（Egr）和细胞死亡的翼成虫盘，苍蝇翅膀的上皮原基的研究中，我们注意到，垂死的细胞出现短暂的积累在egr 3突变翅盘，提高其吞噬吞噬血细胞受损的可能性。进一步的研究表明，egr 3突变体幼虫的淋巴腺和循环血细胞完全没有NimC1染色，这是吞噬血细胞的标志物。基因组测序揭示了NimC1编码区的突变，这些突变预计会在跨膜结构域之前截短NimC1蛋白，并为NimC染色的缺乏提供了解释。我们在这里报告的工作证明了这些NimC1突变存在于广泛使用的egr 3突变体，其姐妹等位基因egr 1及其亲本菌株Regg1GS 9830中。由于egr 3和egr 1等位基因已被用于免疫和细胞死亡的许多研究中，因此建议重新评估其相关表型。
The process of apoptosis in epithelia involves activation of caspases, delamination of cells, and degradation of cellular components. Corpses and cellular debris are then rapidly cleared from the tissue by phagocytic blood cells. In studies of the Drosophila TNF, Eiger (Egr) and cell death in wing imaginal discs, the epithelial primordia of fly wings, we noticed that dying cells appeared to transiently accumulate in egr3 mutant wing discs, raising the possibility that their phagocytic engulfment by hemocytes was impaired. Further investigation revealed that lymph glands and circulating hemocytes from egr3 mutant larvae were completely devoid of NimC1 staining, a marker of phagocytic hemocytes. Genome sequencing uncovered mutations in the NimC1 coding region that are predicted to truncate the NimC1 protein before its transmembrane domain, and provide an explanation for the lack of NimC staining. The work that we report here demonstrates the presence of these NimC1 mutations in the widely used egr3 mutant, its sister allele, egr1, and its parental strain, Regg1GS9830. As the egr3 and egr1 alleles have been used in numerous studies of immunity and cell death, it may be advisable to re-evaluate their associated phenotypes.
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DOI：
10.1126/science.1258236
发表时间：
2014-12-05
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Meyer SN;Amoyel M;Bergantiños C;de la Cova C;Schertel C;Basler K;Johnston LA
通讯作者：
Johnston LA
影响因子：
11.8
作者：
Alpar L;Bergantiños C;Johnston LA
通讯作者：
Johnston LA
影响因子：
4.6
作者：
Makhijani, Kalpana;Alexander, Brandy;Brueckner, Katja
通讯作者：
Brueckner, Katja
影响因子：
14.9
作者：
Attrill H;Falls K;Goodman JL;Millburn GH;Antonazzo G;Rey AJ;Marygold SJ;FlyBase Consortium
通讯作者：
FlyBase Consortium
DOI：
10.1073/pnas.94.11.5691
发表时间：
1997-05-27
影响因子：
11.1
作者：
Milan, M;Campuzano, S;GarciaBellido, A
通讯作者：
GarciaBellido, A