Promoter polymorphisms in genes involved in porcine myogenesis influence their transcriptional activity.
Promoter polymorphisms in genes involved in porcine myogenesis influence their transcriptional activity.
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DOI：
10.1186/s12863-014-0119-2
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发表时间：
2014-11-07
期刊：
影响因子：
2.9
通讯作者：
Valentini A
中科院分区：
文献类型：
--
作者：
Bongiorni S;Tilesi F;Bicorgna S;Iacoponi F;Willems D;Gargani M;D'Andrea M;Pilla F;Valentini A
成功的肉类生产和选择改善肉质是动物生产的主要目标之一。肉质性状在猪中具有重要的经济意义，然而，其潜在的遗传性质非常复杂。因此，提高猪肉产量在很大程度上取决于识别和研究遗传变异如何有助于调节基因表达。启动子是基因调控中的关键区域，因为它们具有与转录调控因子结合的多个基序。因此，这些区域的多态性可能会深刻影响RNA水平，从而影响蛋白质合成。在这项研究中，我们报告的单核苷酸多态性（SNPs）的启动子区域的候选基因参与的发展，细胞分化和肌肉生长的猪鉴定。我们在卡塞塔那和大白色品种中鉴定了属于成肌调节因子（MRF）基因家族（成肌分化基因，MYOD 1）和属于生长和分化因子（GDF）基因家族（肌生成抑制素基因，Myostatin，GDF 8）的基因的启动子区中的SNP。本研究的目的是调查启动子的多态性是否会影响这些基因的转录活性。为了这个目的，我们在体外评估了荧光素酶报告基因luc 2活性的功能活性，该活性由携带不同启动子单倍型的两个构建体驱动。我们测试了MYOD 1基因中G302 A（U12574）转换对启动子效率的影响。我们确定了两种变体的转录效率差异。携带A的构建体的更强活性在C2 C12中更明显。由MYOD 1-A等位基因变体驱动的荧光素酶表达显示出3.8倍的转录活性增加。我们研究了GDF 8基因启动子区的两个单倍型变体（AY 527152）的活性。在CHO和C2 C12培养细胞中，单倍型-1（A435-A447-A879）上调报告基因的表达，使其增加两倍，因此推测为GDF 8基因的表达。MYOD 1-A等位变异体在体外可上调MYOD 1基因的表达。此外，我们可以评估不同的反应，在体外基因表达根据细胞类型用于转染的结构，表明MyoD激活是由成肌细胞的具体机制。本文的在线版本（doi：10.1186/s12863-014-0119-2）包含补充材料，可供授权用户使用。
Success of meat production and selection for improvement of meat quality is among the primary aims in animal production. Meat quality traits are economically important in swine; however, the underlying genetic nature is very complex. Therefore, an improved pork production strongly depends on identifying and studying how genetic variations contribute to modulate gene expression. Promoters are key regions in gene modulation as they harbour several binding motifs to transcription regulatory factors. Therefore, polymorphisms in these regions are likely to deeply affect RNA levels and consequently protein synthesis. In this study, we report the identification of single nucleotide polymorphisms (SNPs) in promoter regions of candidate genes involved in development, cellular differentiation and muscle growth in Sus scrofa. We identified SNPs in the promoter regions of genes belonging to the Myogenic Regulatory Factors (MRF) gene family (the Myogenic Differentiation gene, MYOD1) and to Growth and Differentiation Factors (GDF) gene family (Myostatin gene, MSTN, GDF8), in Casertana and Large White breeds. The purpose of this study was to investigate if polymorphisms in the promoters could affect the transcriptional activity of these genes. With this aim, we evaluated in vitro the functional activity of the luciferase reporter gene luc2 activity, driven by two constructs carrying different promoter haplotypes. We tested the effects of the G302A (U12574) transition on the promoter efficiency in MYOD1 gene. We ascertained a difference in transcription efficiency for the two variants. A stronger activity of the A-carrying construct is more evident in C2C12. The luciferase expression driven by the MYOD1-A allelic variant displayed a 3.8-fold increased transcriptional activity. We investigated the activity of two haplotype variants (AY527152) in the promoter of GDF8 gene. The haploptype-1 (A435-A447-A879) up-regulated the expression of the reporter gene by a two-fold increase, and hence presumably of the GDF8 gene, in both CHO and C2C12 cultured cells. In vitro the MYOD1-A allelic variant could up-regulate the expression of MYOD1 gene. Additionally, we could assess a different response of in vitro gene expression according to cell type used to transfect constructs, suggesting that MyoD activation is regulated by mechanisms that are specific of myoblasts. The online version of this article (doi:10.1186/s12863-014-0119-2) contains supplementary material, which is available to authorized users.
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