Adapting a Low-Cost and Open-Source Commercial Pipetting Robot for Nanoliter Liquid Handling.
Adapting a Low-Cost and Open-Source Commercial Pipetting Robot for Nanoliter Liquid Handling.
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采用低成本、开源的商用移液机器人进行纳升液体处理。
DOI：
10.1177/2472630320973591
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发表时间：
2021-06
期刊：
影响因子：
2.7
通讯作者：
Kelly RT
中科院分区：
文献类型：
--
作者：
Councill EEAW;Axtell NB;Truong T;Liang Y;Aposhian AL;Webber KGI;Zhu Y;Cong Y;Carson RH;Kelly RT
Low-volume liquid handling capabilities in bioanalytical workflows can dramatically improve sample processing efficiency and reduce reagent costs, yet many commercial nanoliter liquid handlers cost tens of thousands of dollars or more. We have successfully adapted a low-cost and open-source commercial pipetting robot, the Opentrons OT-1, to accurately aspirate and dispense nanoliter volumes. Based on fluorescence measurements, the modified OT-1 was able to reproducibly transfer 50 nL of water with less than 3% measurement error and 5% coefficient of variation (CV). For 15 nL transfers, the volume measurements indicated less than 4% error and 4% CV. We applied this platform to the preparation of low-nanogram proteomic samples for liquid chromatography–mass spectrometry analysis, demonstrating that the modified OT-1 is an effective platform for nanoliter liquid handling. At a total materials cost of less than $6000, including the commercial liquid handler and all modifications, this system is also far less expensive than other platforms with similar capabilities, placing automated nanoliter handling within reach of a far broader scientific community.
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影响因子：
16.6
作者：
Piehowski, Paul D.;Zhu, Ying;Burnum-Johnson, Kristin E.
通讯作者：
Burnum-Johnson, Kristin E.
影响因子：
--
作者：
Griffith, Dylan;Northwood, Roger;Bath, Colin
通讯作者：
Bath, Colin
影响因子：
7.4
作者：
Zhu Y;Podolak J;Zhao R;Shukla AK;Moore RJ;Thomas GV;Kelly RT
通讯作者：
Kelly RT
影响因子：
2.5
作者：
Weikart, Christopher M.;Breeland, Adam P.;Maurer, Brian R.
通讯作者：
Maurer, Brian R.
DOI：
10.1002/anie.201802843
发表时间：
2018-09-17
期刊：
Angewandte Chemie (International ed. in English)
影响因子：
--
作者：
Zhu Y;Clair G;Chrisler WB;Shen Y;Zhao R;Shukla AK;Moore RJ;Misra RS;Pryhuber GS;Smith RD;Ansong C;Kelly RT
通讯作者：
Kelly RT