Proteomic profiling of serum extracellular vesicles identifies diagnostic markers for echinococcosis.
Proteomic profiling of serum extracellular vesicles identifies diagnostic markers for echinococcosis.
复制标题
DOI：
10.1371/journal.pntd.0010814
复制
发表时间：
2022-10
影响因子：
3.8
通讯作者：
中科院分区：
文献类型：
--
作者：
棘球蚴病是由棘球蚴后绦虫引起的一种寄生虫病。这种疾病潜伏期长，诊断不足，部分原因是缺乏有效的早期诊断方法。采用液相色谱-质谱联用技术，对大肠杆菌血清来源的细胞外囊泡进行了分析。多室疟原虫感染的小鼠，并确定了三个寄生虫来源的蛋白，硫氧还蛋白过氧化物酶1（TPx-1），过渡性内质网ATP酶（TER ATP酶），和14 - 3 - 3，被连续释放的寄生虫在感染期间通过EV进入血清。用ELISA法检测TPx-1和TER-ATP酶，对实验性鼠包虫病和人包虫病的诊断效果均优于对照组。TERATP酶和TPx-1可作为判断患者预后的指标。本研究发现TER ATP酶和TPx-1作为棘球蚴病有前途的诊断候选者的潜力。棘球蚴病又称包虫病，是一种被忽视的人畜共患病。泡状棘球蚴病和囊状棘球蚴病的病原体分别是多房棘球蚴和广义细粒棘球蚴。泡状棘球蚴病主要在北方地区流行，而囊状棘球蚴病则分布于世界各地。这种疾病的潜伏期长达5至10年，并且在很大程度上诊断不足，部分原因是缺乏有效的早期诊断方法。近年来，已经认识到EV在细胞间通信和诊断中的新兴作用。在这里，我们采用蛋白质组学的方法来确定寄生虫来源的蛋白质在血清EV从E。多房感染的小鼠，并评估其诊断价值。这项研究通过发现两种鉴定的蛋白质，TERATPAs和TPX-1，表明EV在鉴定诊断候选物中的作用。第一个结果表明其诊断和预后价值在实验鼠和人类包虫病。
Echinococcosis is a parasitic disease caused by the metacestodes of Echinococcus spp. The disease has a long latent period and is largely underdiagnosed, partially because of the lack of effective early diagnostic approaches. Using liquid chromatography-mass spectrometry, we profiled the serum-derived extracellular vesicles (EVs) of E. multilocularis-infected mice and identified three parasite-origin proteins, thioredoxin peroxidase 1 (TPx-1), transitional endoplasmic reticulum ATPase (TER ATPase), and 14-3-3, being continuously released by the parasites into the sera during the infection via EVs. Using ELISA, both TPx-1 and TER ATPase were shown to have a good performance in diagnosis of experimental murine echinococcosis as early as 10 days post infection and of human echinococcosis compared with that of control. Moreover, TER ATPase and TPx-1 were further demonstrated to be suitable for evaluation of the prognosis of patients with treatment. The present study discovers the potential of TER ATPase and TPx-1 as promising diagnostic candidates for echinococcosis. Echinococcosis, also known as hydatid disease, is one of the neglected zoonotic diseases. Echinococcus multilocularis and Echinococcus granulosus sensu latu are the causative agents responsible for alveolar echinococcosis and cystic echinococcosis, respectively. Alveolar echinococcosis is mainly endemic in the areas of the northern hemisphere, whereas cystic echinococcosis has a worldwide distribution. The disease has a long latent period of up to 5 to 10 years and is largely underdiagnosed, partially due to the lack of effective early diagnostic approaches. In recent years, an emerging role of EVs in intercellular communication and diagnosis has been recognized. Here, we employed a proteomic approach to identify parasite-derived proteins in the serum EVs from E. multilocularis-infected mice and assessed their diagnostic values. This study signify the role of EVs for the identification of diagnostic candidates by the discovery of two identified proteins, TER ATPas and TPX-1. First results indicate their diagnostic and prognostic values in experimental murine and human echinococcosis.
登录
查看更多内容
小鼠血清中响应多房棘球绦虫感染的循环 miRNA 的表达谱
影响因子：
2.4
作者：
Guo, Xiaola;Zheng, Yadong
通讯作者：
Zheng, Yadong
影响因子：
16
作者：
Marcilla A;Martin-Jaular L;Trelis M;de Menezes-Neto A;Osuna A;Bernal D;Fernandez-Becerra C;Almeida IC;Del Portillo HA
通讯作者：
Del Portillo HA
影响因子：
9.4
作者：
Ito, A;Xiao, N;Vuitton, DA
通讯作者：
Vuitton, DA
影响因子：
--
作者：
Bosque A;Dietz L;Gallego-Lleyda A;Sanclemente M;Iturralde M;Naval J;Alava MA;Martínez-Lostao L;Thierse HJ;Anel A
通讯作者：
Anel A
影响因子：
16
作者：
Jakobsen KR;Paulsen BS;Bæk R;Varming K;Sorensen BS;Jørgensen MM
通讯作者：
Jørgensen MM