Effectiveness of chitosan against mature biofilms formed by food related bacteria.
Effectiveness of chitosan against mature biofilms formed by food related bacteria.
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DOI：
10.3390/ijms12010817
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发表时间：
2011-01-21
影响因子：
5.6
通讯作者：
San Jose C
中科院分区：
文献类型：
--
作者：
Orgaz B;Lobete MM;Puga CH;San Jose C
壳聚糖已被证明具有抑制浮游细胞生长的抗菌特性。然而，人们对其对已经形成的生物膜的影响知之甚少。针对食品工业消毒应用，测试了中分子量 (MMW) 壳聚糖及其酶解产物对四种致病菌株（单核细胞增生李斯特氏菌、蜡样芽孢杆菌、金黄色葡萄球菌和肠沙门氏菌）以及食品腐败菌荧光假单胞菌成熟生物膜的有效性。出乎意料的是，在某些情况下，生物膜的对数减少量高于浮游细胞。暴露于毫米波壳聚糖（1% w/v）一小时导致单增李斯特菌单种成熟生物膜上的活细胞减少 6 个对数，并显着减少（3-5 个对数减少）其他测试生物体的附着种群（金黄色葡萄球菌除外）。对于所有测试的微生物（金黄色葡萄球菌除外），链霉蛋白酶处理的壳聚糖比毫米波壳聚糖更有效，针对蜡样芽孢杆菌的贴壁细胞提供了最佳结果（8 个对数单位）。这些治疗方法为对抗食品工业中的成熟生物膜开辟了新的可能性。
Chitosan has proven antimicrobial properties against planktonic cell growth. Little is known, however, about its effects on already established biofilms. Oriented for application in food industry disinfection, the effectiveness of both medium molecular weight (MMW) chitosan and its enzymatically hydrolyzed product was tested against mature biofilms of four pathogenic strains, Listeria monocytogenes, Bacillus cereus, Staphylococcus aureus and Salmonella enterica, and a food spoilage species, Pseudomonas fluorescens. Unexpectedly, log reductions were in some cases higher for biofilm than for planktonic cells. One hour exposure to MMW chitosan (1% w/v) caused a 6 log viable cell reduction on L. monocytogenes monospecies mature biofilms and reduced significantly (3–5 log reductions) the attached population of the other organisms tested, except S. aureus. Pronase-treated chitosan was more effective than MMW chitosan on all tested microorganisms, also with the exception of S. aureus, offering best results (8 log units) against the attached cells of B. cereus. These treatments open a new possibility to fight against mature biofilms in the food industry.
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影响因子：
6.1
作者：
Je, Jae-Young;Kim, Se-Kwon
通讯作者：
Kim, Se-Kwon
影响因子：
2.2
作者：
Eaton, Peter;Fernandes, Joao C.;Malcata, F. Xavier
通讯作者：
Malcata, F. Xavier
影响因子：
3.5
作者：
Kives, J;Guadarrama, D;SanJose, C
通讯作者：
SanJose, C
影响因子：
5.3
作者：
Fernandes, Joao C.;Tavaria, Freni K.;Malcata, F. Xavier
通讯作者：
Malcata, F. Xavier
影响因子：
2.2
作者：
Bridier, A.;Dubois-Brissonnet, F.;Briandet, R.
通讯作者：
Briandet, R.