Intact and top-down characterization of biomolecules and direct analysis using infrared matrix-assisted laser desorption electrospray ionization coupled to FT-ICR mass spectrometry.
Intact and top-down characterization of biomolecules and direct analysis using infrared matrix-assisted laser desorption electrospray ionization coupled to FT-ICR mass spectrometry.
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DOI：
10.1016/j.jasms.2008.12.003
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发表时间：
2009-04
影响因子：
3.2
通讯作者：
Muddiman DC
中科院分区：
文献类型：
--
作者：
Sampson JS;Murray KK;Muddiman DC
我们报告的实施红外激光到我们以前报道的基质辅助激光解吸电喷雾电离（MALDESI）源与ESI后电离产生多电荷的肽和蛋白质。红外（IR）-MALDESI被证明用于分子量范围从1.2至17 kDa的生物分子的大气压解吸和电离。高分辨率、高质量准确度的单次采集傅里叶变换离子回旋共振（FT-ICR）质谱是从液态和固态肽和蛋白质样品中通过红外激光（2.94 µm）解吸，然后进行ESI后电离生成的。用ESI后电离从固态解吸的马肌红蛋白（17 kDa）的完整和自上而下的分析证明了使用IR-MALDESI偶联FT-ICR质谱的测序能力。碳水化合物和脂质检测通过直接分析牛奶和蛋黄使用UV和IR-MALDESI与最小的样品制备。四类生物大分子（蛋白质，碳水化合物和脂质）中的三种已被电离，并使用MALDESI以最少的样品制备进行检测。还证明了使用还原性β-消除化学从粘蛋白裂解的O-连接聚糖的测序。
We report the implementation of an infrared laser onto our previously reported matrix-assisted laser desorption electrospray ionization (MALDESI) source with ESI post-ionization yielding multiply charged peptides and proteins. Infrared (IR)-MALDESI is demonstrated for atmospheric pressure desorption and ionization of biological molecules ranging in molecular weight from 1.2 to 17 kDa. High resolving power, high mass accuracy single-acquisition Fourier transform ion cyclotron resonance (FT-ICR) mass spectra were generated from liquid-and solid-state peptide and protein samples by desorption with an infrared laser (2.94 µm) followed by ESI post-ionization. Intact and top-down analysis of equine myoglobin (17 kDa) desorbed from the solid state with ESI post-ionization demonstrates the sequencing capabilities using IR-MALDESI coupled to FT-ICR mass spectrometry. Carbohydrates and lipids were detected through direct analysis of milk and egg yolk using both UV- and IR-MALDESI with minimal sample preparation. Three of the four classes of biological macromolecules (proteins, carbohydrates, and lipids) have been ionized and detected using MALDESI with minimal sample preparation. Sequencing of O-linked glycans, cleaved from mucin using reductive β-elimination chemistry, is also demonstrated.
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影响因子：
15
作者：
Kelleher, NL;Lin, HY;McLafferty, FW
通讯作者：
McLafferty, FW
影响因子：
7.4
作者：
Cody, RB;Laramée, JA;Durst, HD
通讯作者：
Durst, HD
DOI：
10.1073/pnas.93.14.7003
发表时间：
1996-07-09
影响因子：
11.1
作者：
Berkenkamp, S;Karas, M;Hillenkamp, F
通讯作者：
Hillenkamp, F
影响因子：
3.7
作者：
Little, Mark W.;Laboy, Jorge;Murray, Kermit K.
通讯作者：
Murray, Kermit K.
影响因子：
4.2
作者：
Rezenom, Yohannes H.;Dong, Jianan;Murray, Kermit K.
通讯作者：
Murray, Kermit K.