Release of extracellular vesicles containing small RNAs from the eggs of Schistosoma japonicum.
Release of extracellular vesicles containing small RNAs from the eggs of Schistosoma japonicum.
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日本血吸虫卵释放含有小RNA的细胞外囊泡
DOI：
10.1186/s13071-016-1845-2
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发表时间：
2016-11-08
影响因子：
3.2
通讯作者：
Pan W
中科院分区：
文献类型：
--
作者：
Zhu S;Wang S;Lin Y;Jiang P;Cui X;Wang X;Zhang Y;Pan W
背景：日本血吸虫是一种引起人类血吸虫病的寄生性扁形虫。分泌的细胞外囊泡（EV）在病原体-宿主界面中起着关键作用。已有的研究表明，S.日本血吸虫蠕虫可以释放含有microRNA（miRNA）的EV，其可以将其货物转移到哺乳动物细胞并调节受体细胞中的基因表达。组织诱捕卵通常被认为是血吸虫病严重病理学的主要贡献者;然而，在这个关键阶段宿主和寄生虫之间的相互作用在很大程度上仍然未知。日本血吸虫卵体外分离EV。透射电子显微镜（TEM）分析用于确认由卵产生的囊泡是基于大小和形态的EV。通过Solexa技术分析从EV提取的总RNA以确定miRNA谱。通过共聚焦显微镜分析哺乳动物细胞对EV的体外内化。采用实时荧光定量PCR（qRT-PCR）检测感染小鼠原代肝细胞中EV相关miRNA的表达。日本虫卵TEM分析证实，纳米大小的囊泡存在于培养基中。RNA-seq分析表明，卵源性EV含有小的非编码RNA（sncRNA）群体，包括miRNA，表明在宿主操纵中的潜在作用。这项研究进一步表明，Hepa 1 -6，一种鼠肝细胞系，内化了纯化的EV及其货物miRNA，这些miRNA在感染S.结论：日本血吸虫卵在体外可释放含miRNA的EVs，EVs可将其运载物转运至受体细胞。这些结果证明了S.在寄生物-宿主界面处的日本血吸虫卵EV。
Background:Schistosoma japonicum is a parasitic flatworm that causes human schistosomiasis. Secreted extracellular vesicles (EVs) play a key role in pathogen-host interfaces. Previous studies have shown that S. japonicum adult worms can release microRNA (miRNA)-containing EVs, which can transfer their cargo to mammalian cells and regulate gene expression in recipient cells. Tissue-trapped eggs are generally considered the major contributor to the severe pathology of schistosomiasis; however, the interactions between the host and parasite in this critical stage remain largely unknown.Methods:The culture medium for S. japonicum eggs in vitro was used to isolate EVs. Transmission electron microscopy (TEM) analysis was used to confirm that vesicles produced by the eggs were EVs based on size and morphology. Total RNA extracted from EVs was analyzed by Solexa technology to determine the miRNA profile. The in vitro internalization of the EVs by mammalian cells was analyzed by confocal microscopy. The presence of EVs associated miRNAs in the primary hepatocytes of infected mice was determined by quantitative real-time PCR (qRT-PCR).Results:EVs were isolated from the culture medium of in vitro cultivated S. japonicum eggs. TEM analysis confirmed that nanosized vesicles were present in the culture medium. RNA-seq analysis showed that the egg-derived EVs contained small non-coding RNA (sncRNA) populations including miRNAs, suggesting a potential role in host manipulation. This study further showed that Hepa1-6, a murine liver cell line, internalized the purified EVs and their cargo miRNAs that were detectable in the primary hepatocytes of mice infected with S. japonicum.Conclusions:Schistosoma japonicum eggs can release miRNA-containing EVs, and the EVs can transfer their cargo to recipient cells in vitro. These results demonstrate the regulatory potential of S. japonicum egg EVs at the parasite-host interface.
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影响因子：
4
作者：
Silverman, Judith Maxwell;Clos, Joachim;Reiner, Neil E.
通讯作者：
Reiner, Neil E.
DOI：
10.1038/nri3622
发表时间：
2014-03
期刊：
Nature reviews. Immunology
影响因子：
--
作者：
通讯作者：
--
影响因子：
16
作者：
Marcilla A;Martin-Jaular L;Trelis M;de Menezes-Neto A;Osuna A;Bernal D;Fernandez-Becerra C;Almeida IC;Del Portillo HA
通讯作者：
Del Portillo HA
影响因子：
14.9
作者：
Mathivanan S;Fahner CJ;Reid GE;Simpson RJ
通讯作者：
Simpson RJ
DOI：
10.1073/pnas.0804549105
发表时间：
2008-07-29
影响因子：
11.1
作者：
Mitchell, Patrick S.;Parkin, Rachael K.;Tewari, Muneesh
通讯作者：
Tewari, Muneesh