Fe-phyllosilicate redox cycling organisms from a redox transition zone in Hanford 300 Area sediments.
Fe-phyllosilicate redox cycling organisms from a redox transition zone in Hanford 300 Area sediments.
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DOI：
10.3389/fmicb.2013.00388
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发表时间：
2013
影响因子：
5.2
通讯作者：
Roden EE
中科院分区：
文献类型：
--
作者：
Benzine J;Shelobolina E;Xiong MY;Kennedy DW;McKinley JP;Lin X;Roden EE
从美国华盛顿东部汉福德300区地下氧化还原过渡带中富集分离出能够还原或氧化含铁层状硅酸盐矿物中结构铁的微生物。采用常规和原位“i-chip”富集策略。一种Fe（III）还原锗（G. Bremensis菌株R1，δ变形菌）和六个Fe（II）层状硅酸盐氧化分离物，它们来自α变形菌（慢生根瘤菌菌株22、is 5和in 8 p8）、β变形菌（钩虫贪铜菌菌株A5-1、搅拌脱氯单胞菌菌株is 5）和放线菌（类诺卡氏菌属菌株in 31）。共和党Bremensis分离物通过用NAu-2蒙皂石的氧化形式作为电子受体氧化乙酸盐而生长。的Fe（II）-氧化剂生长的化学还原蒙皂石作为能源与硝酸盐作为电子受体的氧化。慢生根瘤菌菌株也能进行黑云母的好氧氧化。这是第一次报告的恢复Fe（II）-氧化Nocardioides，到目前为止，只有一个其他的Fe（II）-氧化慢生根瘤菌是已知的。分离株的16 S rRNA基因序列与汉福德300沉积物和地下水克隆文库中发现的序列相似，表明这些生物可能存在并在原位活跃。分离株的全基因组测序正在进行中，其结果将使细胞外页硅酸盐铁氧化还原代谢机制的比较基因组分析，并促进技术的发展，以检测沉积物中微生物页硅酸盐铁氧化还原循环相关基因的存在和表达。
Microorganisms capable of reducing or oxidizing structural iron (Fe) in Fe-bearing phyllosilicate minerals were enriched and isolated from a subsurface redox transition zone at the Hanford 300 Area site in eastern Washington, USA. Both conventional and in situ “i-chip” enrichment strategies were employed. One Fe(III)-reducing Geobacter (G. bremensis strain R1, Deltaproteobacteria) and six Fe(II) phyllosilicate-oxidizing isolates from the Alphaproteobacteria (Bradyrhizobium japonicum strains 22, is5, and in8p8), Betaproteobacteria (Cupriavidus necator strain A5-1, Dechloromonas agitata strain is5), and Actinobacteria (Nocardioides sp. strain in31) were recovered. The G. bremensis isolate grew by oxidizing acetate with the oxidized form of NAu-2 smectite as the electron acceptor. The Fe(II)-oxidizers grew by oxidation of chemically reduced smectite as the energy source with nitrate as the electron acceptor. The Bradyrhizobium isolates could also carry out aerobic oxidation of biotite. This is the first report of the recovery of a Fe(II)-oxidizing Nocardioides, and to date only one other Fe(II)-oxidizing Bradyrhizobium is known. The 16S rRNA gene sequences of the isolates were similar to ones found in clone libraries from Hanford 300 sediments and groundwater, suggesting that such organisms may be present and active in situ. Whole genome sequencing of the isolates is underway, the results of which will enable comparative genomic analysis of mechanisms of extracellular phyllosilicate Fe redox metabolism, and facilitate development of techniques to detect the presence and expression of genes associated with microbial phyllosilicate Fe redox cycling in sediments.
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影响因子：
14.9
作者：
Ludwig, W;Strunk, O;Schleifer, KH
通讯作者：
Schleifer, KH
影响因子：
4.4
作者：
Chaudhuri, SK;Lack, JG;Coates, JD
通讯作者：
Coates, JD
影响因子：
5.1
作者：
Lin, Xueju;Kennedy, David;Konopka, Allan
通讯作者：
Konopka, Allan
影响因子：
11.4
作者：
Lee, Ji-Hoon;Fredrickson, James K.;Phillips, Jerry L.
通讯作者：
Phillips, Jerry L.
影响因子：
4.4
作者：
Lin, Xueju;Kennedy, David;Konopka, Allan
通讯作者：
Konopka, Allan