Differential expression and localization of dentin matrix protein 1 (DMP1) fragments in mouse submandibular glands.
Differential expression and localization of dentin matrix protein 1 (DMP1) fragments in mouse submandibular glands.
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DOI：
10.1007/s10735-012-9464-5
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发表时间：
2013-04
影响因子：
3.2
通讯作者：
Qin C
中科院分区：
文献类型：
--
作者：
Li C;Xie X;Wang X;Sun Y;Liu P;Chen L;Qin C
牙本质基质蛋白1（dentin matrix protein 1，DMP 1）是骨和牙齿形成的重要调控因子。除矿化组织外，DMP 1还在非矿化组织如肾、脑和唾液腺中表达。一些研究表明，DMP 1的表达在癌性腺体中显著升高，而关于DMP 1在这些腺体组织中的表达和定位模式的细节仍然在很大程度上未知。本研究采用多种方法系统分析了DMP 1在小鼠颌下腺中的表达和定位。结果表明，尽管DMP 1在雌性和雄性小鼠SMG中均有表达，但在颗粒曲管（GCT）出现后，雄性小鼠SMG中DMP 1的mRNA水平高于雌性小鼠。在小鼠SMG中，DMP 1主要以46 kDa C-末端片段和37 kDa N-末端片段的形式存在。C-末端片段主要定位于腺泡和导管细胞的细胞核，而N-末端片段仅限于导管细胞的细胞质。本研究发现DMP 1在雄性小鼠GCT中表达，这是一个不同于以往报道的新发现。总的来说，差异定位模式的DMP 1片段表明，不同形式的DMP 1可能发挥不同的作用，在SMG。
It has been demonstrated that dentin matrix protein 1 (DMP1) is an essential regulator in the formation of bone and tooth. In addition to the mineralized tissues, DMP1 is also expressed in the non-mineralized tissues such as kidney, brain and salivary glands. Some studies have shown that the expression of DMP1 is significantly elevated in cancerous glands, while details about the expression and localization patterns of DMP1 in these glandular tissues still remain largely unknown. In this study, with multiple approaches, we systematically analyzed the expression and localization of DMP1 in mouse sub-mandibular glands (SMGs). The results showed that although DMP1 was expressed in both female and male mouse SMGs, the mRNA levels of DMP1 in male mice were higher than those in female mice after the appearance of granular convoluted tubule (GCT). In mouse SMGs, DMP1 was primarily present as the 46 kDa C-terminal fragment and the 37 kDa N-terminal fragment. The C-terminal fragment was mainly localized in the nuclei of acinar and ductal cells, while the N-terminal fragment was restricted to the cytoplasm of ductal cells. This study showed the expression of DMP1 in the GCT of male mice, a novel finding different from the result of previous reports. Collectively, the differential localization patterns of DMP1 fragments indicate that different forms of DMP1 may play distinct roles in the SMGs.
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影响因子：
7.6
作者：
Gericke A;Qin C;Sun Y;Redfern R;Redfern D;Fujimoto Y;Taleb H;Butler WT;Boskey AL
通讯作者：
Boskey AL
DOI：
10.1016/j.bbrc.2012.07.037
发表时间：
2012-08-03
影响因子：
3.1
作者：
Siyam A;Wang S;Qin C;Mues G;Stevens R;D'Souza RN;Lu Y
通讯作者：
Lu Y
影响因子：
4.8
作者：
Narayanan, K;Ramachandran, A;George, A
通讯作者：
George, A
影响因子：
7.6
作者：
Feng, JQ;Huang, H;Mishina, Y
通讯作者：
Mishina, Y
影响因子：
2.9
作者：
Fisher, LW;Fedarko, NS
通讯作者：
Fedarko, NS