lncRNA 1700101O22Rik and NONMMUG030480.1 Are Not Essential for Spermatogenesis in Mice.
lncRNA 1700101O22Rik and NONMMUG030480.1 Are Not Essential for Spermatogenesis in Mice.
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lncRNA 1700101O22Rik 和 NONMMUG030480.1 对于小鼠精子发生不是必需的
DOI：
10.3390/ijms23158627
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发表时间：
2022-08-03
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
许多睾丸特异性lncRNA在精子发生的后期，特别是精子发生中高度表达。然而，它们在男性生育中的功能和潜在机制在很大程度上是未知的。在此，我们筛选了两个在睾丸中高表达的lncRNA，1700101 O22 Rik（O22 Rik）和NONMMUG030480.1（NM 480），以研究在精子发生中的作用。这两种睾丸特异性lncRNA主要在次级精母细胞到圆形精子细胞中表达，表明它们可能参与精子发生。表型分析表明，O22 Rik或NM 480的缺失不影响睾丸和附睾的发育及精子发生。这些结果在年轻和中年雄性小鼠中得到证实。此外，野生型（WT）和KO小鼠之间的精子形态和其他参数（包括浓度和活力）无显著差异。生育力试验显示，与WT对照组相比，O22 Rik KO小鼠的窝仔数显著较低。虽然O22 Rik在精子发生中没有发挥显著作用，但在分子水平上，其周围基因的表达受到显著干扰。GM 32773降低;然而，在KO小鼠中，GM 32828升高。总之，lncRNA O22 Rik和NM 480不是小鼠精子发生所必需的。
Many testis-specific lncRNAs are highly expressed in late spermatogenesis, especially in spermiogenesis. However, their functions and the underlying mechanisms in male fertility are largely unknown. Here, we screened two highly expressed lncRNAs, 1700101O22Rik (O22Rik) and NONMMUG030480.1 (NM480) in testes, to investigate the roles in spermatogenesis using lncRNA knockout (KO) mouse generated by CRISPER/Cas9 technology. Both testis-specific lncRNAs were mainly expressed from secondary spermatocytes to round spermatids, suggesting that they might be involved in spermiogenesis. Phenotypic analysis showed that the deletion of O22Rik or NM480 did not affect the development of testis and epididymis or spermatogenesis. These results were confirmed in both young and middle-aged male mice. In addition, there was no significant difference in sperm morphology and other parameters including concentration and motility between wild type (WT) and KO mice. Fertility tests showed that litter size was significantly lower in O22Rik KO mice compared with WT controls. Although O22Rik did not exert dramatic roles in spermatogenesis, on molecular levels, its surrounding gene expression was disturbed significantly. Gm32773 was decreased; however, Gm32828 was increased in KO mice. In conclusion, lncRNA O22Rik and NM480 are not individually essential for spermatogenesis in mice.
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