Genetic Diversification of Starch Branching Enzymes during Maize Domestication and Improvement.
Genetic Diversification of Starch Branching Enzymes during Maize Domestication and Improvement.
复制标题
玉米驯化和改善期间淀粉分支酶的遗传多样化。
DOI：
10.3390/genes14051068
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发表时间：
2023-05-11
期刊：
影响因子：
3.5
通讯作者：
Li, Pengcheng
中科院分区：
文献类型：
--
作者：
Li, Qi;Yang, Tiantian;Rui, Wenye;Wang, Houmiao;Wang, Yunyun;Yang, Zefeng;Xu, Chenwu;Li, Pengcheng
关键词：
阐明淀粉糊化和凝胶化特性的遗传基础对于提高玉米品质及其作为饲料和工业原料的利用至关重要。在玉米中，ZmSBE基因编码淀粉生物合成途径中的重要淀粉分支酶。在这项研究中，我们重新测序的基因组序列ZmSBEI，ZmSBEIIa，ZmSBEIIb和ZmSBEIII在三个线称为335个自交系，68个地方品种线，和32个大刍草线。核苷酸多态性和单倍型多样性分析揭示了玉米驯化和改良过程中ZmSBEI、ZmSBEIIa、ZmSBEIIb和ZmSBEIII选择模式的差异。对玉米自交系进行标记-性状关联分析，共检测到22个显著位点，其中18个SNP位点和4个indel位点与3个淀粉理化性状显著相关。在3个品系中检测了两个变异体（SNP 17249 C和SNP 5055 G）的等位基因频率。ZmSBEIIb的SNP 17249 C在大刍草系中的频率最高，其次是地方品种，自交系最低，而ZmSBEIII的SNP 5055 G在3个品系中的频率差异不显著。这些结果表明，ZmSBE基因在玉米淀粉理化性质的表型变异中起着重要作用。本研究中检测到的遗传变异可用于开发改良玉米淀粉品质的功能标记。
Elucidating the genetic basis of starch pasting and gelatinization properties is crucial for enhancing the quality of maize and its utility as feed and industrial raw material. In maize, ZmSBE genes encode important starch branching enzymes in the starch biosynthesis pathway. In this study, we re-sequenced the genomic sequences of ZmSBEI, ZmSBEIIa, ZmSBEIIb, and ZmSBEIII in three lines called 335 inbred lines, 68 landrace lines, and 32 teosinte lines. Analyses of nucleotide polymorphisms and haplotype diversity revealed differences in the selection patterns of ZmSBEI, ZmSBEIIa, ZmSBEIIb, and ZmSBEIII during maize domestication and improvement. A marker–trait association analysis of inbred lines detected 22 significant loci, including 18 SNPs and 4 indels significantly associated with three maize starch physicochemical properties. The allele frequencies of two variants (SNP17249C and SNP5055G) were examined in three lines. The frequency of SNP17249C in ZmSBEIIb was highest in teosinte lines, followed by landrace lines, and inbred lines, whereas there were no significant differences in the frequency of SNP5055G in ZmSBEIII among the three lines. These results suggest that ZmSBE genes play an important role in the phenotypic variations in the starch physicochemical properties in maize. The genetic variants detected in this study may be used to develop functional markers for improving maize starch quality.
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DOI：
10.1007/978-1-59745-251-9_17
发表时间：
2009-01-01
期刊：
BIOINFORMATICS FOR DNA SEQUENCE ANALYSIS
影响因子：
--
作者：
Rozas, Julio
通讯作者：
Rozas, Julio
影响因子：
11.6
作者：
Yamasaki, M;Tenaillon, MI;McMullen, MD
通讯作者：
McMullen, MD
影响因子：
5.8
作者：
Bradbury, Peter J.;Zhang, Zhiwu;Buckler, Edward S.
通讯作者：
Buckler, Edward S.
影响因子：
7
作者：
Li X;Zhu C;Yeh CT;Wu W;Takacs EM;Petsch KA;Tian F;Bai G;Buckler ES;Muehlbauer GJ;Timmermans MC;Scanlon MJ;Schnable PS;Yu J
通讯作者：
Yu J
影响因子：
30.8
作者：
Jiao, Yinping;Zhao, Hainan;Lai, Jinsheng
通讯作者：
Lai, Jinsheng