Sample-to-answer palm-sized nucleic acid testing device towards low-cost malaria mass screening.
Sample-to-answer palm-sized nucleic acid testing device towards low-cost malaria mass screening.
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DOI：
10.1016/j.bios.2018.05.019
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发表时间：
2018-09-15
影响因子：
12.6
通讯作者：
Guan W
中科院分区：
文献类型：
--
作者：
Choi G;Prince T;Miao J;Cui L;Guan W
疟疾筛查和治疗的有效性在很大程度上取决于能否以低成本获得高度敏感和特异性的疟疾检测。我们报告了一种用于疟疾现场检测的实时荧光核酸检测设备，具有自动化和可扩展的样品制备能力。该装置由紧凑型分析仪和一次性微流体试剂光盘组成。寄生虫 DNA 样品制备和随后的实时 LAMP 检测无缝集成在单个微流体光盘上，由节能的非离心机磁场相互作用驱动。每个圆盘包含四个并行测试单元，可以配置为四个相同的测试或四个特定物种的测试。当配置为特定物种测试时，它可以识别两种最威胁生命的疟疾物种（恶性疟原虫和间日疟原虫）。 NAT 设备能够在 50 分钟的周转时间内同时处理四个样本。全血的检测限约为 0.5 个寄生虫/μl，足以检测无症状的寄生虫携带者。灵敏度、特异性、成本和可扩展的样品制备相结合表明，实时荧光 LAMP 设备对于现场疟疾筛查特别有用。
The effectiveness of malaria screening and treatment highly depends on the low-cost access to the highly sensitive and specific malaria test. We report a real-time fluorescence nucleic acid testing device for malaria field detection with automated and scalable sample preparation capability. The device consists a compact analyzer and a disposable microfluidic reagent compact disc. The parasite DNA sample preparation and subsequent real-time LAMP detection were seamlessly integrated on a single microfluidic compact disc, driven by energy efficient non-centrifuge based magnetic field interactions. Each disc contains four parallel testing units which could be configured either as four identical tests or as four species-specific tests. When configured as species-specific tests, it could identify two of the most life-threatening malaria species (P. falciparum and P. vivax). The NAT device is capable of processing four samples simultaneously within 50 min turnaround time. It achieves a detection limit of ~0.5 parasites/μl for whole blood, sufficient for detecting asymptomatic parasite carriers. The combination of the sensitivity, specificity, cost, and scalable sample preparation suggests the real-time fluorescence LAMP device could be particularly useful for malaria screening in the field settings.
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影响因子：
4
作者：
Dormond, L.;Jaton, K.;de Valliere, S.;Genton, B.;Greub, G.
通讯作者：
Greub, G.
在疟疾流行环境中对疟疾寄生虫血症的高度敏感性检测：在乌干达偏远诊所中新的环路介导的等温扩增试剂盒的性能。
DOI：
10.1093/infdis/jit184
发表时间：
2013-08-15
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Hopkins H;González IJ;Polley SD;Angutoko P;Ategeka J;Asiimwe C;Agaba B;Kyabayinze DJ;Sutherland CJ;Perkins MD;Bell D
通讯作者：
Bell D
影响因子：
3.7
作者：
Katevatis, Constantinos;Fan, Andy;Klapperich, Catherine M.
通讯作者：
Klapperich, Catherine M.
影响因子：
3
作者：
Britton S;Cheng Q;McCarthy JS
通讯作者：
McCarthy JS
影响因子：
9.4
作者：
Jani, Ilesh V.;Meggi, Bindiya;Peter, Trevor F.
通讯作者：
Peter, Trevor F.