The salivary effector protein Sg2204 in the greenbug Schizaphis graminum suppresses wheat defence and is essential for enabling aphid feeding on host plants.
The salivary effector protein Sg2204 in the greenbug Schizaphis graminum suppresses wheat defence and is essential for enabling aphid feeding on host plants.
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绿虫禾谷裂霉 (Schizaphis graminum) 中的唾液效应蛋白 Sg2204 抑制小麦防御，对于蚜虫以寄主植物为食至关重要
DOI：
10.1111/pbi.13900
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发表时间：
2022-11
影响因子：
13.8
通讯作者：
Chen, Julian
中科院分区：
文献类型：
--
作者：
Zhang, Yong;Liu, Xiaobei;Francis, Frederic;Xie, Haicui;Fan, Jia;Wang, Qian;Liu, Huan;Sun, Yu;Chen, Julian
关键词：
蚜虫向寄主植物细胞分泌多种唾液效应物，通过调节植物防御来促进侵染。禾本科裂蚜（Schizaphis graminum）是一种重要的谷类蚜虫。然而，该菌分泌的效应物仍未被表征。本文利用转录组和蛋白质组学分析，从禾本科葡萄球菌的水样唾液中鉴定出76种唾液蛋白。其中，一种推测的唾液效应物Sg2204在蚜虫取食阶段显著上调，并且Sg2204在烟叶中短暂过表达可抑制BAX或INF1诱导的细胞死亡。通过荧光假单胞菌III型分泌系统将Sg2204输送到小麦中，EtAnH抑制模式触发免疫（PTI）相关的胼质沉积。将Sg2204注入小麦叶片后，小麦茉莉酸（JA）‐和水杨酸（SA）‐相关防御基因的转录水平显著下调，JA和SA的含量也显著降低。此外，取食表达Sg2204的小麦显著增加了小麦黑穗病菌的体重和繁殖力，促进了蚜虫的韧皮部取食。通过基于喷雾的纳米载体介导的透皮dsRNA递送系统有效地沉默了Sg2204。此外，Sg2204沉默的蚜虫诱导了更强的小麦防御反应，并对蚜虫的取食行为、存活和繁殖力产生了负面影响。使用纳米载体传递的dsRNA沉默四种蚜虫的Sg2204同源物也显著降低了蚜虫在寄主植物上的表现。因此，我们的研究发现，S. graminum的唾液效应物Sg2204通过抑制小麦防御来促进寄主敏感性，这也可以被认为是一个很有希望的控制蚜虫的RNAi靶点。
Summary Aphids secrete diverse repertoires of salivary effectors into host plant cells to promote infestation by modulating plant defence. The greenbug Schizaphis graminum is an important cereal aphid worldwide. However, the secreted effectors of S. graminum are still uncharacterized. Here, 76 salivary proteins were identified from the watery saliva of S. graminum using transcriptome and proteome analyses. Among them, a putative salivary effector Sg2204 was significantly up‐regulated during aphid feeding stages, and transient overexpression of Sg2204 in Nicotiana benthamiana inhibited cell death induced by BAX or INF1. Delivering Sg2204 into wheat via the type III secretion system of Pseudomonas fluorescens EtAnH suppressed pattern‐triggered immunity (PTI)‐associated callose deposition. The transcript levels of jasmonic acid (JA)‐ and salicylic acid (SA)‐associated defence genes of wheat were significantly down‐regulated, and the contents of both JA and SA were also significantly decreased after delivery of Sg2204 into wheat leaves. Additionally, feeding on wheat expressing Sg2204 significantly increased the weight and fecundity of S. graminum and promoted aphid phloem feeding. Sg2204 was efficiently silenced via spray‐based application of the nanocarrier‐mediated transdermal dsRNA delivery system. Moreover, Sg2204‐silenced aphids induced a stronger wheat defence response and resulted in negative impacts on aphid feeding behaviour, survival and fecundity. Silencing of Sg2204 homologues from four aphid species using nanocarrier‐delivered dsRNA also significantly reduced aphid performance on host plants. Thus, our study characterized the salivary effector Sg2204 of S. graminum involved in promoting host susceptibility by suppressing wheat defence, which can also be regarded as a promising RNAi target for aphid control.
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影响因子：
4.1
作者：
Bruce, TJA;Martin, JL;Wadhams, LJ
通讯作者：
Wadhams, LJ
影响因子：
5.3
作者：
Chakraborty, Joydeep;Sen, Senjuti;Das, Sampa
通讯作者：
Das, Sampa
DOI：
10.1073/pnas.1407687111
发表时间：
2014-06-17
影响因子：
11.1
作者：
Chaudhary, Ritu;Atamian, Hagop S.;Kaloshian, Isgouhi
通讯作者：
Kaloshian, Isgouhi
DOI：
10.1094/mpmi-01-14-0018-r
发表时间：
2014-07
期刊：
Molecular plant-microbe interactions : MPMI
影响因子：
--
作者：
Elzinga DA;De Vos M;Jander G
通讯作者：
Jander G
PSTha5a23，来自专性生物营养病原体条锈菌 f 的候选效应子。
影响因子：
5.1
作者：
Cheng, Yulin;Wu, Kuan;Kang, Zhensheng
通讯作者：
Kang, Zhensheng