Robust growth of Escherichia coli.
Robust growth of Escherichia coli.
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DOI：
10.1016/j.cub.2010.04.045
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发表时间：
2010-06-22
期刊：
影响因子：
--
通讯作者：
Jun S
中科院分区：
文献类型：
--
作者：
Wang P;Robert L;Pelletier J;Dang WL;Taddei F;Wright A;Jun S
对细胞生长的定量研究在我们对从细胞周期到衰老的广泛主题的理解中产生了许多基本的见解。特别重要的是生长率，它的恒定代表着生物体的生理稳定状态。然而，最近的研究表明，对于不对称和对称分裂的生物体，细菌细胞在指数增长过程中的延长率随着复制年龄的增加而累积下降，这意味着“稳定状态”的种群由从来不处于稳定增长状态的单个细胞组成。为了解决这一看似矛盾的观察结果，我们使用了一种微流控设备来研究大肠杆菌细胞的长期生长和分裂模式，该设备旨在跟踪特定生殖年龄的大量细胞的稳定生长和分裂。我们对105个单个细胞的分析表明，继承同一极点的母细胞在数百代人中的生长具有显着的稳定性。我们进一步证明，大肠杆菌的死亡不是纯粹随机的，而是累积损害的结果。我们的结论是，与迄今研究的所有其他衰老模型系统不同，大肠杆菌具有与细胞死亡脱钩的强大生长机制。
The quantitative study of the cell growth has led to many fundamental insights in our understanding of a wide range of subjects from cell cycle to senescence. Of particular importance is the growth rate, whose constancy represents a physiological steady state of an organism. Recent studies, however, suggest that the rate of elongation during exponential growth of bacterial cells decreases cumulatively with replicative age for both asymmetrically and symmetrically dividing organisms, implying that a “steady-state” population consists of individual cells that are never in a steady state of growth. To resolve this seeming paradoxical observation, we studied the long-term growth and division patterns of Escherichia coli cells by employing a microfluidic device designed to follow steady state growth and division of a large number of cells at a defined reproductive age. Our analysis of ~105 individual cells reveals a remarkable stability of growth of the mother cell inheriting the same pole for hundreds of generations. We further show that death of E. coli is not purely stochastic but is the result of accumulating damages. We conclude that E. coli, unlike all other aging model systems studied to date, has a robust mechanism of growth that is decoupled from cell death.
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影响因子：
7.5
作者：
Henderson, Kiersten A.;Gottschling, Daniel E.
通讯作者：
Gottschling, Daniel E.
DOI：
10.1099/00221287-19-3-592
发表时间：
1958-01-01
期刊：
JOURNAL OF GENERAL MICROBIOLOGY
影响因子：
--
作者：
SCHAECHTER, M;MAALOE, O;KJELDGAARD, NO
通讯作者：
KJELDGAARD, NO
影响因子：
3.2
作者：
Barker, CS;Prüss, BM;Matsumura, P
通讯作者：
Matsumura, P
DOI：
10.1073/pnas.0706115104
发表时间：
2007-11-27
影响因子：
11.1
作者：
Lu, Ting;Shen, Tongye;Hasty, Jeff
通讯作者：
Hasty, Jeff
影响因子：
64.8
作者：
MORTIMER, RK;JOHNSTON, JR
通讯作者：
JOHNSTON, JR